The extracellular matrix (ECM) is a promising biomaterial for numerous applications. Using cells from human donors and synthetic carbohydrate derivatives, functionalized ECM modified with dienophiles, such as cyclopropenes, was produced by metabolic glycoengineering. cover feature highlights the application of inverse-electron-demand Diels-Alder reaction to “click” variable groups R in cell-fri...

A simple and efficient metal-free methodology for the regioselective synthesis of 1,4-disubstituted-1,2,3-triazoles has been developed by applying a novel inverse electron-demand-1,3-dipolar cycloaddition approach. The practical one-pot metal-free strategy can be accomplished with various alkylidene malononitriles and aromatic azides in the presence of base.

We give an efficient algorithm for computing a Cournot equilibrium when the producers are confined to integers, the inverse demand function is linear, and costs are quadratic. The method also establishes existence constructively. We use our characterization to discuss the multiplicity of integer Cournot equilibria and their relationship to the real Cournot equilibrium.

A concise asymmetric synthesis of (-)-halenaquinone is described. The synthesis features a diastereoselective Heck cyclization to set a quaternary center as well as a novel intramolecular inverse-electron-demand Diels-Alder reaction involving a vinyl quinone. The synthesis is highly convergent and features a minimal amount of protecting group manipulations.

A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels-Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.

In this Communication, we report a system in which an achiral Lewis acid (activating the diene) works in concert with a chiral nucleophile (dienophile) to effect the first highly enantio- and regioselective catalytic inverse electron demand Diels-Alder [4 + 2] cycloaddition reaction to form biologically active quinoxalinones from ketene enolates and o-benzoquinone diimides in good to excellent ...

Increased competition for viewers’ time is threatening the viability of publicservice broadcasters (PSBs) around the world. Changing regulations regarding advertising minutes might increase revenues, but little is known about the structure of advertising demand. To address this problem, we collect a unique dataset on monthly impacts (quantities) and prices of UK television channels between 2002...

The scanning ntuple classifier is an efficient and accurate classifier for handwriting recognition. One of the major difficulties in implementing this scheme is its demand for a very large memory space, thus making it unsuitable for resource constrained systems such as embedded applications. This paper proposes some modifications to the basic sntuple algorithm which eliminates the necessity of ...