The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment ...

background: potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. potyviruses can be transmitted through plant sap, seeds and many aphid species. due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. objectives: this study compared the efficiencies of two couples of primers in thei...

To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amp...