Sugarcane-associated nematodes (Saccharum spp.) can reduce productivity up to 50%. Through the survey, it was possible identify main that occur in a certain region as tool for designing best management and control strategies. The aim of this study characterize population associated with sugarcane North Central, Pioneiro Northwest mesoregion state Paraná, Brazil, quantify nematode genera crop sp...

A genetic approach was cited for species detection of the ameba genus Naegleria using allozyme electrophoresis to characterize the trophozoite stage of three strains of Naegleria fowleri isolated from patients with primary amebic meningoencephalitis, five thermophilic (45 degrees C) Naegleria spp isolated from natural water sources in the Taling Chan district, and a reference control strain, Na...

The levels of a number of enzymes of carbohydrate metabolism were assayed in the cancerous and morphologically normal (host) portions of livers from nine primary malignant hepatoma cases obtained at autopsy. The levels of these enzymes in two normal human adult and two fetal livers were also determined. The activities of phosphoglucomutase, fructose 1,6-diphosphatase and a-glycerophosphate dehy...

We separated and measured amylase isoenzymes in the serum and urine of 3036 normal persons by electrophoresis on a thin layer of polyacrylamide gel. We wished to establish the normal pattern of these isoenzymes and to evaluate the usefulness of this method of electrophoresis in clinical diagnosis. Results for patients with hyper- or hypofunctioning pancreas and salivary glands suggested that es...

We analytically and clinically evaluated Abbott's IMx assay for creatine kinase (CK) isoenzyme MB (CK-MB) in serum. Over a 1-year period, the method was more specific but less precise than catalytic isoenzyme measurements by electrophoresis or immunoinhibition. Sera from different individuals without electrophoretic evidence of CK-MB but containing macro CK type 1 (n = 20), mitochondrial CK (n ...

The isoenzymesof alkalinephosphatase(ALP; EC 3.1.3.1) present in the .sera of 116 patients with hepatobiliary disease were studied by electrophoresis on cellulose acetate. Individual liver isoenzyme bandswere identified an quantitated by densitometnc scanning. The occurrence and magnitude of increase of the isoenzyme bands of liver ALP (a2 alone or a1 and cx2) are related to diagnosis. Of the c...

We describe a simple, rapid immunoaffinity procedure for purifying the MB isoenzyme of creatine kinase. Immunoaffinity gel is prepared by linking a monoclonal antibody ("Conan-MB"), specific for this isoenzyme, to Sepharose 4B. Heart tissue is homogenized and fractionated with 40-70% saturated ammonium sulfate before it is applied to the immunoaffinity gel. CK-MB activity, retained on the gel, ...

There are many reports on the appearance of a typical isoenzyme of alkaline phosphatase (EC 3.1.3.1; API)2 in serum of patients suffering from biliary obstruction. According to the characteristics observed in various studies, this API has variouslybeen termed “high molecular weight” (1), “origin,” “stationary,” “particulate” (2), “bile,” “cholestatic,” “pathological biliary” (3, 4), and “koinoz...

Acid phosphatase isoenzyme (AcP-II) from leaves of germinating seeds of vigna radiata (mung beans) was partially purified by CM-Cellulose chromatoghraphy, gelfiltration on Ultrogel AcA 44 and Con A-Sepharose affinity chromatoghraphy. The specific activity of 25U/ mg of protein was obtained with recovery of 4 %. The enzyme showed a purification by a factor of 86. Gel filtration experiment and so...