Transplantation of a human lymphoma to the cheek pouches of unconditioned, adult golden hamsters resulted in permanently transplantable tumors which grow progressively and kill their hosts. This tumor system, GW-478, has a hamster-specific láclate dehydrogenase isoenzyme mobility pattern and both human and hamster glucose 6-phosphate dehyrogenase enzyme profiles when propagated in the hamster ...

Colorectal mucosa from patients with colorectal carcinoma was compared with rectal mucosa from a control group. It was found that the LDH isoenzyme pattern of uninvolved mucosa proximal and distal to the carcinoma differed from that of normal tissue and resembled that of carcinoma tissue except in the case of the uninvolved mucosa proximal to the carcinoma of the sigmoid colon, which did not di...

The isoenzymesof alkalinephosphatase(ALP; EC 3.1.3.1) present in the .sera of 116 patients with hepatobiliary disease were studied by electrophoresis on cellulose acetate. Individual liver isoenzyme bandswere identified an quantitated by densitometnc scanning. The occurrence and magnitude of increase of the isoenzyme bands of liver ALP (a2 alone or a1 and cx2) are related to diagnosis. Of the c...

The isoenzyme pattern of enolase was examined in the aqueous humour and serum of patients with retinoblastoma (10 aqueous, 8 sera), malignant melanoma (4 aqueous, 25 sera), and normal subjects undergoing cataract surgery (25 aqueous, 30 sera). The assay we used allowed assessment of all three major isoenzymes, including the gamma gamma isoenzyme (neurone-specific enolase). No enolase was detect...

We previously reported an automatic method for quantitative analysis of schistocytes or fragmented red cells using an automatic hematology analyzer, XE-2100. In the study reported here, we evaluated the accuracy of this detection method in patients with thrombotic microangiopathy (TMA). A follow-up study was performed on 14 patients with two types of TMA, thrombotic thrombocytopenic purpura or ...

HeLa TCRC-1, is a monophenotypic cell line for the production of the carcinoplacental Regan isoenzyme of alkaline phosphatase. It has been previously reported that, when this cell line is grown in immunosuppressed rats, there is an alteration of phenotypic expression in that an isoenzyme that is referred to as oncoamnion (FL) becomes the dominant enzyme form. This report describes the isoenzyme...

The separation of N-acetyl-beta-D-hexosaminidase isoenzymes from human tissues is used in the diagnosis and differential diagnosis of GM(2) gangliosidosis, since in type 1 the A isoenzyme is deficient and in type 2 both the A and B isoenzymes are deficient. Peripheral blood leucocytes are commonly used for these investigations, and the present study demonstrates that, in addition to these two i...

BY CELLULOSE ACETATE OR ACRYLAMIDE GEL ELECTROPHORESIS IT IS POSSIBLE TO SEPARATE THESE ALKALINE PHOSPHATASE ISOENZYMES FROM SERUM: [anode] fast liver, slow liver, placenta/Regan, bone, intestine, bile [cathode]. Heat or chemical inhibition can confirm the differentiation. Normal adult serum always contains slow-liver isoenzyme, and sometimes bone isoenzyme: the latter is always present in seru...

A case of malignant schwannoma of the sciatic nerve is described associated with hepatic dysfunction in the absence of hepatic metastases. An elevated serum alkaline phosphatase activity was present with an isoenzyme pattern indicating hepatic involvement. These abnormalities disappeared after extirpation of the tumour. The patient is well, with no evidence of metastases, over two years later. ...

Study of the activities of hyaluronidase, lactate dehydrogenase isoenzyme-X and the dehydrogenases of sorbitol, alpha-glycerophosphate, glucose-6-phosphate, malate, glyceraldehyde-3-phosphate and isocitrate in the testes of mice of different ages showed that the changes were correlated with the stages of spermatogenic cell differentiation. The first 4 enzymes had activities which were low in ne...