The adult-plant leaf rust resistant gene Lr22a confers broadly effective resistance against the fungal pathogen Puccinia triticina Eriks. (Pt) in wheat that has not been extensively utilized cultivars. objective of this study was to develop robust functional molecular markers using sequence facilitate integration breeding programmes. coding used identify isolated SNPs and four kompetitive allel...

The contributions of three proteinase genes (rgpA, rgpB, and kgp) to the virulence of Porphyromonas gingivalis W50 were investigated in the murine periodontitis model. Mice were orally inoculated with eight doses (1 x 10(10) cells per dose) of rgpA, rgpB, kgp, rgpA rgpB, or rgpA rgpB kgp isogenic mutants, and the level of alveolar bone loss, immune response induced, and number of bacterial cell...

Doubled haploids (DHs) are essential founders for breeding via isogenic line production. We determined the optimal timing of first and second (HST1 HST2) double heat shock treatment DH production in willow gudgeon (Gnathopogon caerulescens). Gynogenesis was induced by fertilisation with UV-irradiated sperm (20 mJ/cm2). Each HST performed at 40.5°C 1 min. In experiment I, HST1 applied 23–31 min ...

Background: The optimum maturity duration for maximum grain yield of non-photosensitive rice (Oryza sativa) under direct-seeded condition in the tropics was studied. Information on such studies conducted using near-isogenic lines are lacking. Change with explained components. Methods: experiment dry zone Sri Lanka over two seasons namely and wet during 2021 2022. Non-photosensitive that varying...

Twenty-eight heavy-chain variable (VH1) region genes and the immunoglobulin (IgM) heavy-chain constant region of an isogenic Xenopus hybrid, X. laevis/X. gilli, LG7, have been sequenced. The LG7 clone represents the first Xenopus hybrid that is homozygous for the IgH locus. The VH1 family was specifically investigated because VH1 genes are used by the antibodies produced during the Xenopus anti...

Abstract: Heritability shows the degree of resemblance between relatives. Heritability is the most important genetic parameter on which different breeding strategy depends. The knowledge of heritability is a prerequisite for the formulation of breeding plans on scientific lines. There is need to know the heritability of different characters which are used for selection of parents for future bre...

Efficient gene targeting in embryonic stem cells requires that modifying DNA sequences are identical to those in the targeted chromosomal locus. Yet, there is a paucity of isogenic genomic clones for human cell lines and PCR amplification cannot be used in many mutation-sensitive applications. Here, we describe a novel method for the direct cloning of genomic DNA into a targeting vector, pRTVIR...