SCOPE This study aims to use a combination of biochemical and biophysical methods to derive greater mechanistic understanding of the interactions between tea polyphenols and porcine pancreatic α-amylase (PPA). METHODS AND RESULTS The interaction mechanism was studied through fluorescence quenching (FQ), differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC) and com...

The enthalpy of unfolding (DeltauH) of carbonic anhydrase II was determined by titrating the protein with acid and measuring the heat using isothermal titration calorimetry (ITC) in the temperature range of 5 to 59 degrees C. By combining the ITC results with our previous findings by differential scanning calorimetry (DSC) in the temperature range of 39 to 72 degrees C, the DeltauH dependence o...

Characterization of the thermodynamics of DNA- drug interactions is a very useful part in rational drug design. Isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC) and UV melting experiments have been used to analyze the multivalent (intercalation plus minor groove) binding of the antitumor antibiotic chartreusin to DNA. Using DNA UV melting studies in the presence o...

The trp RNA-binding attenuation protein (TRAP) functions in many bacilli to control the expression of the tryptophan biosynthesis genes. Transcription of the trp operon is controlled by TRAP through an attenuation mechanism, in which competition between two alternative secondary-structural elements in the 5' leader sequence of the nascent mRNA is influenced by tryptophan-dependent binding of TR...

The binding behaviour of differently substituted diamide axle molecules to Hunter/Vögtle tetralactam macrocycles was studied with a combination of NMR titration, isothermal titration calorimetry (ITC) experiments and calculations employing density functional theory (DFT), along with dispersion-corrected exchange-correlation functionals. Guests with alkyl or alkenyl chains attached to the diamid...

Thermodynamic studies on protein adsorption onto chromatographic surfaces mainly focus the molecular level interaction between proteins and ligands. Yet, not much attention is given to study of polymer grafted ligand architecture effect thermodynamic parameters, nor relation parameters directly obtained parameters. These relations are needed in order confer meaning ease future data interpretati...

Ion exchange chromatography is a widely used method for purification of all types biomolecules in current biotechnological downstream processes. Knowledge on the binding behavior proteins provides valuable insight understanding molecular mechanisms protein interactions biological context. However, thermodynamic parameters such as enthalpy and entropy changes that characterize adsorption are sti...

A new shape-memory polymer is presented, in which both the stable phase as well switching unit consist of two different metal complexes. Suitable ions, simultaneously form labile complexes with histidine and ones terpyridine ligands, are identified via isothermal titration calorimetry (ITC) measurements. Different copolymers synthesized, contain butyl methacrylate main monomer metal-binding lig...

Isothermal titration calorimetry (ITC) was utilised to investigate suitability of the technique to determine the stoichiometry and thermodynamics of the interactions that occur between a commonly used chemotherapeutic drug, namely doxorubicin, and a polymer bead-based drug delivery embolisation system (DC Bead™). Six temperatures were selected for drug-polymer titrations (293-313 K) and in all ...