Leucocyte alkaline phosphatase (LAP) is a granulocyte enzyme whose concentration varies in disease states. In order to determine whether the pattern of expression is altered in leukaemic granulocytes, we have analysed the LAP isozyme pattern of a series of normal subjects and patients with various haematological diseases. Electrophoretic patterns of partially purified LAP samples were determine...

The effects of Gö-6976, a Ca(2+)-dependent protein kinase C (PKC) isozyme inhibitor, and rottlerin, a PKC-delta isozyme/calmodulin (CaM)-dependent kinase III inhibitor, on responses to vasopressor agents were investigated in the feline pulmonary vascular bed. Injections of angiotensin II, norepinephrine (NE), serotonin, BAY K 8644, and U-46619 into the lobar arterial constant blood flow perfusi...

Leaf extracts of 27 `Yuzu' and related acid citrus cultivars were analyzed using polyacrylamide gel electrophoresis for isozyme variation of glutamate oxaloacetate transaminase (GOT) and shikimate dehydrogenase (SDH). SDH yielded 12 different isozyme phenotypes and six cultivars were discriminated by this enzyme alone. GOT produced 10 different isozyme phenotypes and four cultivars were separat...

Forebrain and cerebellar Type II Ca2+/calmodulin-dependent protein kinases have different subunit compositions. The forebrain holoenzyme, characterized in our laboratory, is a 650-kDa holoenzyme composed of 50-kDa alpha-subunits and 60-kDa beta-subunits assembled in approximately a 3:1 ratio (Bennett, M. K., Erondu, N. E., and Kennedy, M. B. (1983) J. Biol. Chem. 258, 12735-12744). The cerebell...

Antibodies to cytochrome P-450 isozyme 3a, the ethanol-inducible isozyme in rabbit liver, were used to determine the role of this enzyme in the microsomal oxidation of alcohols and the p-hydroxylation of aniline. P-450 isozymes, 2, 3b, 3c, 4, and 6 did not crossreact with anti-3a IgG as judged by Ouchterlony double diffusion, and radioimmunoassays indicated a crossreactivity of less than 1%. Gr...

ALTERATIONS IN BIOCHEMICAL COMPONENTS IN MESTA PLANTS: Yellow vein mosaic disease of mesta (kenaf, Hibiscus cannabinus L.; and roselle, H. sabdariffa L.) is a new entrant to the disease scenario and it is associated with a novel monopartite Begomovirus. Changes in different biochemical parameters in diseased mesta plants were observed as compared to healthy ones. Isozyme pattern and assays of d...

Glutamate dehydrogenase becomes density labeled through the incorporation of deuterium and (15)N when detached oat leaves (Avena sativa var. Fulghum) are incubated in the presence of ammonia. The enzyme has been isolated by means of DEAE-cellulose chromatography, ammonium sulfate precipitation, isopycnic equilibrium centrifugation, and disc electrophoresis from leaves fed l-methionine-(35)S. Ra...

To test the possibility that individuals with and without plasma cholinesterase Cs isozyme have differences in neuromuscular sensitivity to succinylcholine chloride, we ~ramined the effects of succinylcholine in these two groups of patients. Sera from 491 adult patients were examined for presence of the plasma cholinesterase Cs isozyme by use o f electrophoresis with polyacrylamide ; 24 were posi-

Thesis Abstract A greenhouse experiment was conducted in order to assess the possible effects of Nostoc muscorum (2 g/ kg soil fresh pellets) on growth and some metabolic activities Trigonella foenum gracum at 30, 60 days yield under different concentrations CuSO4. Application a mixture with Cu significantly increased all parameters, photosynthetic pigments activity, soluble insoluble nitrogen ...

OBJECTIVE To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, R stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. METHODS N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied. RESULTS EST electrophoresis show...