A kinetic colorimetric assay procedure is described for measuring lactate dehydrogenase activity, in which the reduction of NAD is coupled to the reduction of a tetrazolium salt, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride (INT), with phenazine methosulfate serving as an intermediate electron carrier. The molar absorptivity of the formazan of INT was found to be 19.3 X io at 50...

The aim of this study is to explore the capability of subcritical methanol to reduce the acidity of naphthenic acids and to determine reaction kinetics for large-scale reactor design.The experiments were carried out in a 25 mL autoclave reactor (China) at temperatures of 70-120oC, Methanol Partial Pressures (MPPs) of 0.1-1.5 MPa, and reaction times of 0-60 min. The total...