We report the first case of post-kala-azar dermal leishmaniasis due to Leishmania infantum in a human immunodeficiency virus type 1-infected patient in Australia. Molecular characterization of the isolate was performed using PCR restriction fragment length polymorphism targeting both repetitive sequences from Leishmania nuclear DNA and repetitive kinetoplast DNA minicircles for species differen...

Eucaryotic cells contain two complete genetic systems, one based on nuclear DNA and the other on cytoplasmic DNA . The study of these systems has been greatly facilitated by the existence of specific inhibitors . One such inhibitor is the trypanocidal drug, ethidium bromide (Short, Peak, and Watkins, 1953) . Application of the drug to trypanosomes in vitro causes alterations in the structure of...

The effects of acriflavine on two species of Trypanosomatidae, Crithidia luciliae and Trypanosoma mega, have been investigated. It has been observed that kinetoplastic (i.e. mitochondrial) DNA is lost in a high percentage of acriflavine-treated cells. Resting flagellates, from stationary-phase or hemin-deficient cultures, are considerably more resistant to the acridine than are flagellates from...