Abstract Background The prevalence of antimicrobial resistance (AMR) among Gram-negative bacteria is alarmingly high. Reintroduction colistin as last resort treatment in the infections caused by drug-resistant has led to emergence and spread resistance. This study was designed determine drug-resistance beta-lactamase-producing strains Escherichia coli Klebsiella pneumoniae, isolated from clinic...

BACKGROUND & OBJECTIVE Metallo beta lactamase (MBL) producing Pseudomonas aeruginosa have been reported to be important cause of nosocomial infections. The appearance of MBL genes and their spread among bacterial pathogens is a matter of concern with regard to the future of antimicrobial therapy. The present study was undertaken to determine the incidence of MBL producing P. aeruginosa in patie...

Background and objectives: Metallobeta-lactamase production is one of the most important mechanisms of antibiotic resistance. The aim of the present study was to evaluate the antibacterial activity of Stachys lavandulifolia and Quercus brantii on the IMP-type metallo-beta-lactamase-producing Pseudomonas aeruginosa. Methods: This study...

In this study, we have evaluated the efficacy of calcium-EDTA (Ca-EDTA) as an inhibitor of bacterial metalloenzymes, such as metallo-β-lactamase (MBL) and other proteases, in a mouse model of Pseudomonas aeruginosa pneumonia. The simultaneous presence of Ca-EDTA (32 μg/ml) reduced the MICs of imipenem (IPM) in all MBL-producing P. aeruginosa isolates (IMP-1, -2, -7, and -10 and VIM-2) but not n...

background: pseudomonas aeruginosa is the causing agent of many hospital infections and metallo-beta-lactamases (mbl) are being reported with increasing frequency. the aim of this study was to determine the frequency of metallo-&beta-lactamases; (mbl) and vim-1 gene in multidrug-resistant strains of p. aeruginosa isolates and to compare the methods of phenotypic and molecular detection. materia...

The recent increase in gram-negative bacteria coproducing multiple classes of β-lactamases has made it difficult to accurately identify metallo-β-lactamase (MBL) producers. In the present study, six methods for detecting MBL producers were compared using 56 gram-negative bacterial isolates that produce various β-lactamases. Sodium mercaptoacetic acid (SMA) and EDTA were used as inhibitors for a...

Gram-negative bacilli with acquired metallo-beta-lactamase (MBL) production have been increasingly reported in some countries, necessitating their detection. The aim of this study was to evaluate the performance of the Hodge test and those of the imipenem (IPM)-EDTA, ceftazidime (CAZ)-mercaptopropionic acid (MPA), and CAZ-sodium mercaptoacetic acid (SMA) double-disk synergy tests (DDSTs). The e...

OBJECTIVES To analyse the metallo-β-lactamase (MBL) genes and their genetic environments in clinical isolates of Achromobacter xylosoxidans. METHODS From January 2004 to December 2010, four MBL-producing, multidrug-resistant (MDR) A. xylosoxidans strains were isolated and analysed at a tertiary care university hospital in Japan. Species-level identification was confirmed by 16S ribosomal RNA ...

Carbapenems are group of extended-spectrum β-lactam antimicrobials frequently used for treating multidrug-resistant Gram-negative bacilli (GNB) infections. This study aimed at detecting and characterizing carbapenem resistance (CR) genes among GNB isolated from patients treated in neonatal intensive care unit (NICU) Mansoura University Children's Hospital (MUCH), Egypt. It is a prospec...

background :   imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (mbls)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams....