Lactoferrin (LF) and β-lactoglobulin (BLG) are among the protein pairs that exhibit heteroprotein coacervation, a unique and relatively unexamined type of liquid-liquid phase separation (LLPS). In prior work we found that LF and BLG undergo coacervation at highly constrained conditions of pH, ionic strength and protein stoichiometry. The molar stoichiometry in coacervate and supernatant is LF :...

objective(s): candidiasis infection caused by candida albicans has been known as a major problem in patients with immune disorders. the objective of this study was to genotype the c. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (hiv+) with or/and without oropharyngeal candidiasis (opc). materials and methods:a total of 100 c. albicans isolat...

background: the present study was aimed to investigate molecular diversity of echino-coccus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in iran . methods: forty seven human hydatid cysts were collected through surgery from two hospitals in tehran during 2010-2012. to determine the fertility of protoscoleces, the cyst fluids were subjecte...

background: hydatidosis or cystic hydatid disease is one of the most important diseases in human and animals. identification of strains is important for improvement of control and prevention of dis-ease. the aim of this study was to determine the strains isolated from human and domestic animals in ilam province, iran, using pcr-rflp method. methods: respectively, 30 and 4 animal and human hydat...

b-lactoglobulin (coded by the b-lg gene) is the major milk whey protein in ruminants. studies have shown that this protein is polymorphic in many breeds of sheep as a result of a single base pair substitution in the b-lg gene that also gives rise to rsai restriction fragment length polymorphism (rflp). blood samples were collected from 391 animals belonging to 5 iranian and 6 russian sheep bree...

We have studied the formation and the stability of grafted block complex coacervate core micelles (C3Ms) in solution and the influence of grafted block C3M coatings on the adsorption of the proteins beta-lactoglobulin, bovine serum albumin, and lysozyme. The C3Ms consist of a grafted block copolymer PAA(21)-b-PAPEO(14) (poly(acrylic acid)-b-poly(acrylate methoxy poly(ethylene oxide)), with a ne...

Objective: To investigate the humoral and cellular immune response against cow’s milk proteins in Behçet’s disease and to distinguish any behaviour during active or inactive disease. Methods: Peripheral blood mononuclear cells from 16 patients and from eight normal controls were cultured in the presence of phytohaemagglutinin (PHA), β-casein, β-lactoglobulin, or chicken egg albumin. Interferon ...

Protein glycation (non-enzymatic glycosylation) and the development of advanced glycation end-products (AGEs) is strongly associated with the pathogenesis of diabetic secondary complications [I ] . Hydroxyl radicals generated from superoxide via the Fenton reaction have been implicated for in virro AGE formation [2]. The term glycoxidation has been coined to emphasise the involvement of metal c...

Temperature jump studies of aqueous @-lactoglobulin have been conducted between pH 3.7 and 2.0, where a reversible dissociation of the protein into two identical subunits has been reported to occur, using methyl orange as an indicator. Two relaxation processes have been observed, both of which can be attributed to intramolecular interactions between the indicator dye and protein rather than to ...