This paper presents the spectral emission and saturation properties of a Lumilass G9 fluorescence glass excited using a 157 nm laser emitting 10 ns duration pulses. This fluorescence glass can be applied in conjunction with a CCD array for beam profiling and divergence measurements. This method can also be used for deriving information on the spatial coherence function of the F2 laser. This con...

Capillary electrophoresis provides very high efficiency separations of biological molecules. Laser-induced fluorescence produces very high sensitivity detection. The combination of the two techniques results in an analytical tool with unprecedented properties: separations with more than a million theoretical plates and detection limits of a few hundred analyte molecules. This paper considers th...

Lanthanides/actinides sorption speciation on minerals and oxides by means of time resolved laser fluorescence spectroscopy (TRLFS), extended X-ray absorption fine structure spectroscopy (EXAFS) and density functional theory (DFT) is reviewed in the field of nuclear disposal safety research. The theoretical aspects of the methods are concisely presented. Examples of recent research results of la...

The interaction of calcite with trivalent europium under recrystallization conditions was studied on the molecular level using site-selective time-resolved laser fluorescence spectroscopy (TRLFS). We conducted batch studies with a reaction time from seven days up to three years with three calcite powders, which differed in their specific surface area, recrystallization rates and impurities cont...

A new Doppler-free laser spectroscopy method is demonstrated that employs modulation of the position of a laser beam rather than the commonly used intensity or polarization modulation. The technique is applicable in saturated absorption as well as fluorescence measurements, as is illustrated in experiments on sodium and iodine lines. A particular feature of the method is that Doppler- and backg...

The results of experimental studies the fluorescence response polylactide foams saturated with rhodamine 6G and composites used for their preparation in region transition from spontaneous to stochastic laser generation are presented. A small increase threshold foamed compared expected one is interpreted as a result contribution waveguide mode propagation polymer matrix.. Keywords: foam, fluores...

Single-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interactions, and dynamics. This is accomplished using ratios that report on the distance between and the...

The use of laser induced fluorescence (LIF) spectroscopy to characterize crude oil has privileges compared to conventional techniques in terms of reducing the size of sample, rapidity of measurements, high sensitivity and simplicity of sample handling and possibility of in situ measurements. The fluorescence radiation emitted from crude oil is the result of the interaction between the aromatic ...

Supplemental Experimental Procedures Confocal UV Laser Scanning Microscopy of Filipin-Sterol Fluorescence Dot-Blot Detection of Filipin-Sterol Fluorescence Analysis of Arabidopsis roots was performed by employing a Nikon Sterols (Sigma) and 4-cholesten-3-one (Sigma) in chloroform at 20 Diaphot 300 inverted microscope, connected to a Nikon RCM 8000 mM were spotted onto Transblot nitrocellulose m...

A pulsed dye laser was used as an excitation source in a fluorescent treponemal antibody absorption (FTA-ABS) test. A high precision in quantitative fluorescence was obtained with this high-power excitation source coupled to an electronic detection system and a storage oscilloscope by standardization of fluorescence evaluation and through elimination of human error. One 0.4-mus pulse exposure w...