Abstract Cryptogenic cerebro-vascular stroke is defined as ischemic without definite etiology in spite of complete work up. It differs from embolic undetermined source (ESUS) which a subgroup including the cardio-embolic sources.We aimed to evaluate left atrial function using two-dimensional speckle tracking echocardiography (2D-STE) possible cause for cryptogenic or predictor subclinical AF.TE...

The opportunistic pathogen Pseudomonas aeruginosa uses intercellular signals to control the density-dependent expression of many virulence factors. The las and rhl quorum-sensing systems function, respectively, through the autoinducers N-(3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone (C(4)-HSL), which are known to positively regulate the transcription of the elastase-...

Abstract Introduction Right ventricular function among β-thalassemia (β-TM) patients is affected by both pulmonary circulation pressure and primary iron deposition on right myocardium. Purpose In our study we aimed to detect the presence of impaired ventricle (RV-PC) coupling applying novel echocardiographic techniques these patients. Methods 47 β-TM (mean age 39.02±8.76 years, 48.9% male sex) ...

In cystic fibrosis (CF), Pseudomonas aeruginosa undergoes intra-strain genotypic and phenotypic diversification while establishing and maintaining chronic lung infections. As the clinical significance of these changes is uncertain, we investigated intra-strain diversity in commonly shared strains from CF patients to determine if specific gene mutations were associated with increased antibiotic ...

A well-designed and properly validated primer produces a specific efficient qPCR assay. The primer’s melting temperature, G-C content, its size, as well the amplicon’s size are principal considerations while designing for this study it was in accordance with MIQE guidelines. Subsequently, designed evaluated prior to analysis verify efficiency precision relative quantification of gene transcript...

N-acetyl glucosamine, the monomer of chitin, is an abundant source of carbon and nitrogen in nature as it is the main component and breakdown product of many structural polymers. Some bacteria use N-acyl-L-homoserine lactone (AHL) mediated quorum sensing (QS) to regulate chitinase production in order to catalyze the cleavage of chitin polymers into water soluble N-acetyl-D-glucosamine (NAG) mon...

Identification of host factors that interact with pathogens is crucial to an understanding of infectious disease, but direct screening for host mutations to aid in this task is not feasible in mammals. The nematode Caenorhabditis elegans is a genetically tractable alternative for investigating the pathogenic bacterium Pseudomonas aeruginosa. A P. aeruginosa toxin, produced at high cell density ...