Leptospirosis is a zoonotic disease with global distribution that caused by pathogenic spirochetes of the genus Leptospira. Accurate diagnosis for differentiation of leptospirosis from other pyrogenic infections prevailing in the same locality and is imperative for proper treatment. Therefore a molecular diagnostic test with high specificity and sensitivity such as PCR is essential. Gene encodi...

The importance of cats in the Leptospira epidemiology is due to the possibility of transferring leptospirosis to wild and domesticated animals. The purpose of this survey was to determine the prevalence of Leptospira infection in shorthair cats in different location of Mashhad, Iran. Totally, 147 blood samples were taken from 42 (28.57%), 52 (35.37%) and 53 (36.05%) households...

ABSTRACT: Leptospirosis is a zoonosis that can infect buffaloes and other mammalians, it’s caused by Gram-negative bacteria of the genus Leptospira spp. The infection occurs through contact with urine or contaminated environment mucous membranes animals, disease has two phases: leptospiremia leptospiruria. objective this study was to determine frequency leptospirosis in state Paraná, Brazil, as...

Over a period of five years (2005–2010) a total of 358 red fox (Vulpes vulpes) serum samples were collected during regular hunting procedures, mostly in the continental part of Croatia. All samples were tested using the microscopic agglutination test (MAT) for the presence of specific antibodies (Ab) to 12 Leptospira spp. pathogenic serovars. Specific antibodies for 11 different Leptospira spp....

Detecção de Leptospira spp. através da reação em cadeia pela polimerase (Pf.R) em sêmen bovino experimentalmente contaminado 1997 1998 CORRESPONDENCE TO: Leonardo José Richtzenhain Departamento de Medicina Preventiva e Saúde Animal Faculdade de Medicina Veterinária e Zootecnia da USP Cidade Universitária Armando de Salles Oliveira. Av. Orlando Marques de Paiva, 87. 05508-900. São Paulo-SP e-mai...

Pathogenic Leptospira spp. cause leptospirosis upon contact with mucosa through wounds or ingestion, leading to headaches, fever, jaundice, kidney or liver failure, or death in about 1.3 million people each year. Here, we present the draft genomes of one L. santarosai isolate and two L. interrogans isolates from Ecuador.

Leptospirosis is an ubiquitous zoonotic infection.It is spread worldwide. Currently the genus Leptospira is classified in 13 genomic species and 4 genomospecies based upon phylogenetic and phenotypic relatedness.The goal of this study was to determine the distribution of aerobic spirochetes Leptospira in surface waters and soils in Guilan province of Iran. A total of 222 fresh water samples wer...

We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.

Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.