For the measurement of lactate dehydrogenase (LDH) isoenzyme biosynthesis a new method is presented which is based on the quantitative hybridization of isoenzyme MI with isoenzyme Hq. After reversible dissociation the formation of active LDH M4 was more rapid and occurred to a greater extent than the formation of LDH Hq. The formation of hybrid isoenzymes from the two subunits resulted in an in...

We characterized a new pathway to induce tolerogenic dendritic cells (DCs) following treatment of human monocyte-derived DCs with proteases from the fungus Aspergillus oryzae (ASP). ASP-treated DCs (ASP-DCs) exhibit a CD80(-)CD83(-)CD86(-)Ig-like transcript (ILT)2(-)ILT3(-)ILT4(+) phenotype, do not secrete cytokines or chemokines, and express tolerogenic markers such as glucocorticoid-induced l...

Previous studies demonstrated an asymmetry of left predominance for mean values soluble leucine aminopeptidase (LeuAP) activity in the frontal cortex (FC) and hypothalamus adult male rats, fluorimetrically analyzed by hydrolysis Leu-β-naphthylamide as a substrate. No asymmetries were observed nine other (L) right (R) regions obtained from rostro-caudally sectioned coronal slices. Neither had in...

Rats were given radioactive r.-leucine by intravenous injection. At times ranging from 30 set to 4 hours after injection, livers were frozen in situ, lyophilized, and separated into nuclear and cytoplasmic fractions in nonaqueous solvents. In each of the fractions thus prepared, the following were determined: (a) concentration of free labeled amino acid, (b) specific activity of the total prote...

Abstract Background To make the right treatment decisions about colorectal cancer (CRC) patients reliable predictive and prognostic data are needed. However, in many cases this is not enough. Some studies suggest that LRIG1 gene ( leucine-rich repeats immunoglobulin-like domains1 ) has implications different kinds of cancers. Methods One hundred two with were retrospectively analyzed for expres...

Shengjimycin is a group of 4"-acylated spiramycins with 4"-isovalerylspiramycin as the major component, produced by recombinant S. spiramyceticus F21 harboring a 4"-O-acyltransferase gene from S. mycarofaciens 1748. A stable bioengineered strain of Streptomyces spiramyceticus WSJ-1 was constructed by integrating the 4"-O-acyltransferase gene (ist) by homologous recombination into the chromosome...