OBJECTIVE To evaluate the effect of low-level laser irradiation on the proliferation and possible nuclear morphological changes of mouse mesenchymal stem cells. METHODS Mesenchymal stem cells derived from bone marrow and adipose tissue were submitted to two applications (T0 and T48 hours) of low-level laser irradiation (660 nm; doses of 0.5 and 1.0 J/cm2). The trypan blue assay was used to ev...

Background: Exposure to ionizing radiation is inevitable. Using of low-level laser therapy (LLLT) stimulates tissue repair and reduces inflammation. The objective of the present study aimed at evaluating the therapeutic efficacy of Helium-Neon (He-Ne) laser in stimulating the reparative processes in the liver of mice after whole body gamma-irradiation (WBγ-I). Materials and Methods: Two hundred...

Low-power, soft, or low-level laser irradiation has been successfully used to provide analgesia in injured or diseased tissues. In this study, we tested the possible antinociceptive effect of laser irradiation when applied to a normal tissue before the onset of a painful stimulus. Male Wistar rats (350-380 g) were used. A 1.5% formalin solution (50 microL s.c., diluted in saline) was injected i...

Effects of phototherapy using low-level lasers depend on irradiation parameters and the type of laser used. The aim of the present study was to evaluate the effect of phototherapy on the proliferation of cultured C2C12 myoblasts under different nutritional conditions using low-level GaAlAs and InGaAlP lasers with different parameters and incubation periods. C2C12 cells cultured in regular and n...

We investigated the effects of Ga-Al-As laser irradiation on the mineralization ability of human dental pulp (HDP) cells and on Smads and bone morphogenetic protein (BMP) production as one mechanism for the transmission of laser photochemical energy to cells. HDP cells in vitro were irradiated once with a Ga-Al-As laser at 1.0 W for 500 s, and calcified nodule formation was assessed by Alizarin...

Several published studies have revealed a unique biological effect in adipocytes following lowlevel laser stimulation. Dr. Rodrigo Neira and coworkers (2000) were able to demonstrate that low-level laser light at 635nm emulsified isolated adipose panicles. Scanning and transmission electron microscopy (SEM and TEM) revealed the collapse of fat-filled adipocytes in panicle arrangements following...

objectives: dental caries and periodontal diseases are caused by infection of teeth and supporting tissues due to complex aggregate of bacteria known as biofilm, firstly colonized by streptococci. the main purpose of this in vitro study was to evaluate the antimicrobial effects of toluidine blue o (tbo) and radachlorin® in combination with a diode laser on the viability of streptococcus mutans....

background: the effectiveness of low power lasers on incisional wound healing, because of confl icting results of previous studies, is uncertain. therefore, the aim of this study was to evaluate the effects of low-level helium-neon (he-ne) laser irradiation on wound healing in rat’s oral mucosa. materials and methods: sixty-four standardized incisions were carried out on the buccal mucosa of 32...

OBJECTIVE To investigate the effect of therapeutic infrared class 3B laser irradiation on skin temperature in healthy participants of differing skin color, age, and gender. BACKGROUND Little is known about the potential thermal effects of Low Level Laser Therapy (LLLT) irradiation on human skin. METHODS Skin temperature was measured in 40 healthy volunteers with a thermographic camera at la...