This study aimed to investigate whether the preovulatory LH surge mechanism is involved in sheep-to-sheep interaction for oestrous synchronisation. For that purpose, anoestrous ewes were inserted with intravaginal progestagen sponges for 13 days to induce ovulation. Upon sponge withdrawal, they were introduced either to 4 rams (n = 6, ram group), or to 4 ewes which were in follicular period (n ...

Angus x Hereford multiparous cows were assigned to four treatments: 1) mastectomized+calf weaned at birth (MCW; n = 7); 2) mastectomized+calf presence restricted to noninguinal contact (MCR; n = 7); 3) mastectomized+unrestricted calf presence (MCP; n = 7); and 4) udder-intact cows+unrestricted calf presence (UICP; n = 8). Except for MCW cows, cow-calf pairs were penned together individually fro...

OBJECTIVE To produce weight for length/height (WLH) percentiles to be used for the screening of growth and assessment of failure to thrive in infancy and early childhood. METHODS The data (2009-2010) of the Anthropometry of Turkish Children aged 0-6 years (ATCA-06) study were used. A cross-sectional study was designed to calculate the WLH references. Reference weight values for each 5-cm LH i...

Effects of Syncro-Mate B (SMB) and(or) short-term calf removal (CR) on pituitary and ovarian responses after gonadotropin releasing hormone (GnRH) were studied in two experiments. In Exp. 1, 24 thin, 2-yr-old, anestrous, Brahman crossbred cows were allotted randomly to a 2 x 2 factorial design involving 27-h CR and SMB. All cows were injected (iv) with 150 /ag GnRH at 24 h after removal of impl...

The reaction of tetranitromethane with the tyrosine residues of the pituitary glycoprotein hormones, luteinizing hormone (LH) and thyroid-stimulating hormone (TSH) has been compared. The total reaction products were inactive if sufficient reagent was used. Although polymer formation is a major reaction, nitrated LH monomer was obtained which was then separated into its a and /3 subunits and a f...

Opiates have been implicated in learned helplessness (LH), a phenomenon known to be related to opiate stress-induced analgesia (SIA). In the present study, we investigated the role of opiates in the induction of LH and SIA under different conditions. Adult female Wistar rats were trained either by receiving 60 inescapable 1-mA footshocks (IS group, N = 114) or by confinement in the shock box (c...

BACKGROUND The objective was to determine the effect of prepartum diets supplemented with rolled canola seed (high in oleic acid) or sunflower seed (high in linoleic acid) on luteinizing hormone (LH) pulsatility and gonadotropin releasing hormone (GnRH)-induced LH release during early postpartum. METHODS Thirty-one pregnant Holstein cows, blocked by body condition score, parity and expected c...

The essential role played by progesterone in the maintenance of pregnancy is unequivocal; however, the effects of progesterone on the secretory patterns of placental and pituitary molecules during the gestation period are not well defined. The objective of this study was to describe pregnancy-associated glycoprotein (PAG) concentrations (measured by RIA-497 and RIA-Pool) in pregnant females wit...

The composition, structure, and biological activity of humic-like substances (HLS) synthesized in the process lignosulfonate conversion for production humic product Lignohumate® (LH) were examined. It is shown that during hydrolytic-oxidative process, transformation raw material accumulation HLS occur. Data on chemical (elemental content, functional groups, FTIR) spectral (absorbance fluorescen...

A method for maintaining primary cultures of ovarian cells in serum-free medium in which the metabolic functions of the androgen-producing cells can be studied is described. Freshly dispersed cells from ovaries of hypophysectomized immature rats contained interstitial cells with specific, high affinity lZ5I-human chorionic gonadotropin (hCG) binding sites which were functionally coupled to aden...