?-Glutamyl transpeptidase (GGT) catalyzes the transfer of glutathione’s ?-glutamyl group and related amides to water, amino acids or peptides, utilizes a conserved Thr residue process its own polypeptide chain into large small subunit that then assemble produce catalytically competent enzyme. In this study, magnetic cross-linked enzyme aggregates (mCLEAs) transpeptidase-specialized variant (N45...

Determining to what extent biophysical characteristics of aggregates affect immunogenicity of therapeutic interferon beta-1b. Three recombinant human interferon beta-1b (rhIFNβ-1b) samples with different levels of aggregates generated by copper oxidation, thermal stress, or left untreated, as well as Avonex(®) drug substance and Betaferon(®) drug product, were injected intraperitoneally in nont...

The popular amyloid hypothesis states that beta-amyloid protein aggregates to form sticky deposits in the brain tissue, causing Alzheimer’s disease. Current research studies propose that oligomeric morphologies of beta-amyloid are the principal pathogenic form of the protein. Through the use of a highly-specified capture enzyme-linked immunosorbent assay, the relative quantity of beta-amyloid o...

An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...