Endothelial lipase (EL) is a new member of the triglyceride lipase gene family previously reported to have phospholipase activity. Using radiolabeled lipid substrates, we characterized the lipolytic activity of this enzyme in comparison to lipoprotein lipase (LPL) and hepatic lipase (HL) using conditioned medium from cells infected with recombinant adenoviruses encoding each of the enzymes. In ...

LPL, lipoprotein lipase; TG, triglyceride; VLDL, very low density lipoproteins; HL, hepatic lipase; PL, pancreatic lipase; EL, endothelial lipase; HSPG, heparin sulfate proteoglycans; cld, combined lipase deficiency; fld, fatty liver dystrophy; LMF, lipase maturation factor; ANGPTL, angiopoietin-like; RAP, receptor-associated protein; APOA5, apolipoprotein A5; CHO, carbohydrate. Articles in Pre...

Our aim was to determine whether the increase in serum pancreatic lipase values, reported in patients with chronic renal failure maintained on haemodialysis, is the result of haemoconcentration by fluid removal during dialysis, or whether it is due to lipase stimulation by endothelial lipoprotein lipase, induced by the heparin used as an anticoagulant. We therefore compared the increases in ser...

Based on longitudinal twin data in women, we have previously demonstrated a genetic influence on changes in lipoprotein risk factors, blood pressure measurements, and body mass index over a decade. The present study examined the linkage between changes in lipoprotein variables and candidate genes encoding the hormone-sensitive lipase (HSL), hepatic lipase (HL), and lipoprotein lipase (LPL). The...

BACKGROUND Interferon therapy has been shown to induce lipid abnormalities. AIM We assessed the effects of interferon-beta on the lipoprotein profile and post-heparin lipase activities in 26 normolipaemic patients with chronic hepatitis C. METHODS Interferon-beta was administered subcutaneously at doses of 6 x 106 U (units) three times a week, over 6 months, and lipoproteins and post-hepari...

We analyzed the molecular defects in the lipoprotein lipase gene of a patient with type I hyperlipidemia suffering from recurrent pancreatitis, indicative for lipoprotein lipase deficiency. Postheparin lipoprotein lipase activity in the patient was decreased by 70%. Direct genomic sequencing revealed compound heterozygosity for two mutation: the well-known Gly188-->Glu and a new Val69-->Leu sub...

to percent of ideal body weight (r = 0.76, P < 0.001 for controls; r = 0.67, P <0.05 for diabetics) and to fat cell size (r = 0.71, P <0.01 for controls; r = 0.85, P <0.01 for diabetics. Postprandial-stimulated insulin secretion was related to diet-induced changes in lipoprotein lipase in control subjects; both were dependent upon the amount of dietary carbohydrate. In contrast, the diabetic pa...

In order to clarify the mechanism of hyperlipemia during pancreatic disorders, investigations on changes of serum lipids, measurements of lipoprotein and number of chylomicra after oral administration of fat (safflower oil or olive oil) and studies on post-heparin lipoprotein lipase were done on patients with chronic pancreatitis and on dogs with experimental pancreatic disorders. The results o...

The effect of cholesteryl oleate on the lipoprotein lipase-catalyzed hydrolysis of trioleoylglycerol was determined in monolayers of egg phosphatidylcholine at a constant surface pressure of 24 mN m-1. The phospholipid monolayers contained 1.0 to 7.5 mol % trioleoylglycerol and various amounts (0 to 20 mol %) of cholesteryl oleate. The initial rates of trioleoylglycerol hydrolysis were determin...

Steady state expression of lipoprotein lipase was compared in abdominal and gluteal subcutaneous adipose tissue of nonobese men and women. In both regions enzyme activity and lipoprotein lipase mRNA levels were significantly higher in women than in men. In men the enzyme activity was higher in abdominal than in gluteal adipose tissue (P less than 0.01) whereas the opposite was observed in women...