A homogenate of epidermal cells isolated from human skin converted arachidonic acid to 12S-hydroxy-5, 8,10,14-eicosatetraenoic acid and 15-hydroxy-5, 8,11,13-eicosatetraenoic acid as the main lipoxygenase products. The production of these hydroxy acids was not stimulated by the addition of 1 mM NADPH required for cytochrome P-450 reaction, but inhibited by 65-75% with 40 microM nordihydroguaiar...

This study aimed to investigate the oxidation profile of wheat germ oil extracted from raw during stabilization with microwave (MW) treatment, and kinetics parameters (free fatty acids (FFA), peroxide value (PV), thiobarbituric acid (TBA), α-tocopherol, lipase (LA) lipoxygenase (LOX) enzymes activities) under different storage conditions. For stabilizing germ, MW was treated at 700 W for three ...

A more simplified method of identification of the tertiary aminopropiophenones formed from various allylbenzene derivatives is reported. By use of combined gas-liquid chromatography and chemical ionization mass spectrometry, one is able to detect and verify the chemical structure of the tertiary aminopropiophenones of elemicin on nanogram quantities in the crude basic urine fraction. By use of ...

The effect of human platelets with deficient lipoxygenase activities on leukotriene B4 (LTB4) synthesis by neutrophils was studied. When arachidonic acid (AA) metabolites obtained from the incubation of washed normal neutrophils and platelets with N-formylmethionylleucylphenylalanine (FMLP), cytochalasin B, and AA were analyzed by reversed-phase high-performance liquid chromatography, the synth...

Localized surface plasmon resonances (LSPR) generated in a particle-film nanocavity enhance electric fields within nanoscale volume. LSPR can also decay into hot carriers, highly energetic species that catalyze photocatalytic reactions molecular analytes located close proximity to metal surfaces. In this study, we examined the intensity of field (near-field) and properties plasmonic nanocavitie...

the anti-senescence biogenic polyamines (pas) putrescine (put), spermidine (spd) and spermine (spm) are involved in plant growth processes and responses to stress. the physiological mechanism of exogenous put to affect the senescence of cut lisianthus flowers during vase life was investigated. fresh cut lisianthus flowers were treated with distilled water (control), 0.5, 1 and 2 mm put and then...

Lipoxygenase(LOX) catalyzes irreversible transfer of oxygen molecule to Arachidonic and Linoleic acid to produce 13 Hydroproxy Octadecadienoic acid. Recent studies showed the involvement of Lipoxygenase products, leukotrienes, in inflammations and Lipoxygenase pathways acts as mediators of early inflammatory events in atherosclerosis. The aim of the present study was purification and characteri...

A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s concentration range of 25-350 μg/ml. The relative standard devi...