BACKGROUND Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in developed countries. NAFLD encompasses a spectrum of diseases, ranging from hepatic steatosis to non-alcoholic steatohepatitis (NASH), cirrhosis, and liver failure. The etiology of NAFLD remains unclear but is thought to relate to increased fatty acid flux within the liver that results in t...

This study aimed to evaluate the effect of dietary protein source (fishmeal, FM; or plant-feedstuffs, PF) and protein/carbohydrate (P/CH) ratio on gilthead seabream appetite regulation intermediary metabolism. Additionally, sampling 5 h after feeding (AF) compared 24 AF was also evaluated. Four isolipidic diets were formulated having as major sources FM PF (20% 80% PF), P/CH ratios 50/10 40/20,...

While radiation hazards, due to free radical generation, present an enormous challenge for biological and medical safety, melatonin is a potent scavenger of a variety of free radicals. The aim of this study was to investigate the radioprotective effect of melatonin against oxidative stress and tissue injury induced by gamma radiation. Rats were subjected to two doses of 2 and 4Gy from cesium-13...

Glucose infusion in rats for 1-4 days results in insulin resistance and increased triglyceride, whole tissue long-chain fatty acyl-CoA (LCA-CoA), and malonyl-CoA content in red skeletal muscle. Despite this, the relation between these alterations and the onset of insulin resistance has not been defined. We aimed to 1) identify whether the changes in these lipids and of diacylglycerol (DAG) prec...

AIM Non-alcoholic fatty liver disease (NAFLD) is commonly diagnosed in patients with obesity and type 2 diabetes mellitus (T2DM), and has been associated with the single nucleotide polymorphism (SNP) rs738409 in the PNPLA3 gene. This association remains to be investigated in African Americans with T2DM, a group at lower risk for hepatic steatosis relative to European Americans with T2DM. METH...

The effects of dietary adenine in fatty liver induced by orotic acid (OA) were studied. Rats were paired-fed 1% OA-supplemented diets with/or without 0.25% adenine or a diet without OA for 10 days. Serum lipid profiles were measured using enzyme assay kits. Lipids of liver tissues were extracted and liver lipid contents were determined. A peach of liver was prepared to determine the activities ...

The metabolism of lipids in the ischemic liver has been examined in the attempt to define the cause of the previously described loss of phospholipid and to determine whether additional alterations occur that may be related to the disturbances in membrane function. With 3 hr of ischemia, 30% of the cellular phospholipid was lost when measured either as phosphate in a lipid extract of the whole l...

background: although several observational and experimental studies have examined the effects of low carbohydrate diets (lcds) on nonalcoholic fatty liver disease (nafld), there are considerable inconsistencies among studies. we summarized the effect of lcds on liver function tests, including intrahepatic lipid content (ihlc), alanine transaminase (alt), aspartate aminotransferases (ast),and ga...

Golgi fractions isolated from rat liver homogenates have been resolved into membrane and content subfractions by treatment with 100 mM Na2CO3 pH 11.3. This procedure permitted extensive extraction of content proteins and lipoproteins, presumably because it caused an alteration of Golgi membranes that minimized the reformation of closed vesicles. The type and degree of contamination of the fract...