Protease widely exists in the digestive tract of animals and humans, playing a very important role in protein digestion and absorption. In this study, a high protease-producing strain Planomicrobium sp. L-2 was isolated and identified from the digestive tract of Octopus variabilis. The strain was identified by physiological and biochemical experiments and 16S rDNA sequences analysis. A protease...

We have developed a culture-based method for determining the production of secreted protease inhibitors. The assay utilizes standard proteolysis detection plates to support microbial growth followed by infiltrating the plate with a protease and subsequently detecting the remaining protein by trichloroacetic acid (TCA) precipitation, or by bromocreosol green (BCG) or Ponseau S (PS) staining. The...

Tracheobronchial mucin samples from control and cystic fibrosis patients were purified by gel filtration chromatography on Sephacryl S-1000 and by density gradient centrifugation. Normal secretions contained high molecular weight (approximately 10(7] mucins, whereas the cystic fibrosis secretions contained relatively small amounts of high molecular weight mucin together with larger quantities o...

Background: Phytocystatins are plants cysteine protease inhibitors (CPIs) that known for their numerous uses in medicine and biotechnology. Methods: Different extraction media which include, Sodium Hydroxide, Hydrochloric acid, Chloride, phosphate buffer distilled Water were used to evaluate flower, leave, root, latex, stem bark of Moringa oleifera inhibitory activity against (Papain enzyme). T...

The seven reviews and the eleven articles in this special issue provide an updated survey of recent research and developments in the ever-growing field of heparin, along with low molecular weight heparins (LMWHs) and glycosaminoglycans (GAGs).

Background The National Patient Safety Agency issued a rapid response report in July 2010 aimed at reducing treatment dose errors with low molecular weight heparins (LMWHs) (1). This states: “incident reports indicate patients renal function is often not taken into consideration when prescribing treatment doses of LMWHs. Not considering renal function was the leading causes of error resulting i...

not received.