The predominant K + channel in human T lymphocytes is Kvl.3, which inactivates by a C-type mechanism. To study assembly of these tetrameric channels in Jurkat, a human T-lymphocyte cell line, we have characterized the formation of heterotetrameric channels between endogenous wild-type (WT) Kvl.3 subunits and heterologously expressed mutant (A413V) Kvl.3 subunits. We use a kinetic analysis of C-...

Previous work in our laboratory has shown that microvascular pericytes sort muscle and nonmuscle actin isoforms into discrete cytoplasmic domains (Herman, I. M., and P. A. DAmore. 1985. J. Cell Biol. 101:43-52; DeNofrio, D., T. C. Hoock, and I. M. Herman. J. Cell. Biol. 109:191-202). Specifically, muscle (ix-smooth) actin is present on the stress fibers while nonmuscle actins (/3 and 3') are lo...

Endocytosis is a process of internalization of extracellular fluid or cell surface receptors via diverse cell surface invaginations, generating an endosomal membrane system (Conner and Schmid 2003, Gruenberg, 2001, Gruenberg and Maxfield 1995, Guha et al. 2003, Mellman et al. 1986, Mukherjee et al. 1997, Sharma et al. 2002). It plays an important role in uptake of nutrients, scavenging of extra...

The notion of (m,n)-ideals of semigroups was introduced by Lajos [13, 14]. Later (m,n) quasi-ideals and (m,n) bi-ideals and generalized (m,n) bi-ideals were studied in various algebraic structures. The notion of a rough set was originally proposed by Pawlak [16] as a formal tool for modeling and processing incomplete information in information systems. Some authors have studied the algebraic pr...

American Journal of Botany 98(10): 1–7, 2011; http://www.amjbot.org/ © 2011 Botanical Society of America Polyploidy is a major source of genetic diversity in plants and may lead to adaptation and phylogenetic diversifi cation ( Grant, 1981 ). In fact, as many as 80% of angiosperm species may have polyploidal origins ( Grant, 1981 ; Masterson, 1994 ; Soltis and Soltis, 1999 ). Cytotypic variatio...