Medicago polymorpha L. is a herbaceous legume that can be useful pasture crop, especially in Mediterranean climates. This study aimed to analyze the genetic variation five populations of M. collected from different eco- geographic regions Tunisia using eight SSR markers. The transferability 112 markers distributed on chromosomes truncatula Gaertn. showed 50 could amplified polymorpha. Among the...

Contour-clamped homogeneous electrophoresis and an embedded-agarose method of sample preparation were combined to carry out an analysis of the chromosome sets of nine strains of Hansenula polymorpha (syn. Pichia angusta). Chromosomal DNA molecules could be separated into a series of bands ranging, approximately, from 650 up to 2,200 kb in size. Polymorphism of the electrophoretic pattern was de...

The TPS1 gene from Hansenula polymorpha, which encodes trehalose-6-phosphate (Tre6P) synthase, has been isolated and characterized. The deletion of TPS1 rendered H. polymorpha cells incapable of trehalose synthesis under conditions where wild-type cells normally accumulate high levels of trehalose. Interestingly, the loss of Tre6P synthase did not cause any obvious growth defects on a glucose-c...

BACKGROUND The methylotrophic yeast, Hansenula polymorpha is an industrially important microorganism, and belongs to the best studied yeast species with well-developed tools for molecular research. The complete genome sequence of the strain NCYC495 of H. polymorpha is publicly available. Some of the well-studied strains of H. polymorpha are known to ferment glucose, cellobiose and xylose to eth...

Bovine follicle-stimulating hormone (bFSH), a pituitary gonadotropin, is a heterodimer hormone that consists of a common alpha-subunit non-covalently associated with the hormone-specific beta-subunit. Unfortunately, expression levels of recombinant bFSH or its subunits are invariably low. We report here the secretory expression of biologically active bFSHalpha and bFSHbeta subunit in the methyl...

We describe the cloning of the Hansenula polymorpha PER1 gene and the characterization of the gene and its product, PER1p. The gene was cloned by functional complementation of a per1 mutant of H. polymorpha, which was impaired in the import of peroxisomal matrix proteins (Pim- phenotype). The DNA sequence of PER1 predicts that PER1p is a polypeptide of 650 amino acids with no significant sequen...

Brazil has a high diversity of flies of the genus Drosophila, and part of this richness is represented by the cardini group. We analyzed the fluctuations in the richness and abundance of this group, in environments that had never previously been studied in the northeastern region of Brazil. Among the 28,204 drosophilids sampled, 1,294 belonged to the cardini group and were represented by D. pol...

Cytogenetic characterization of D. polymorpha was carried out using banding techniques such as C-banding, fluorochrome CMA3 and silver nitrate treatment. The diploid chromosome number of both investigated D. polymorpha forms (typical and albinotic) was the same 2n = 32 (NF = 56). The karyotype consisted of 5 pairs of metacentric, 7 pairs of submetacentric and four pairs of subtelo-acrocentric c...

(KCS).2) Several KCS genes from higher plants have been identified, e.g., Arabidopsis thaliana E4El, CU71I, and KCSI,3-5) Simmondsia chinensis Gojoba) Kes,6) Brcmssica nupus (rape) KCS,'} and Limnanthes dougldsii (meadowfoam) E4E.S) The A4EI gene of A. thaiiana, for example, encodes a seed-specific fatty acid elongase which extends the 18 and 20 carbon acyl chains to 20 and 22, respectively.3) ...

BACKGROUND The thermotolerant methylotrophic yeast Hansenula polymorpha is capable of alcoholic fermentation of xylose at elevated temperatures (45 - 48 degrees C). Such property of this yeast defines it as a good candidate for the development of an efficient process for simultaneous saccharification and fermentation. However, to be economically viable, the main characteristics of xylose fermen...