b ackground: toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. since the aim of this study was to assess the diagnostic utility of recombinant sag1 (rec- sag1) for the detection of toxoplasma -specific igm antibodies in human sera, by an enzyme-linked immunosor...

Automated blood counts from a patient with Waldenström's macroglobulinaemia repeatedly failed critical limit standards set for mean cell haemoglobin concentration and mean cell haemoglobin. Haemoglobin estimation was higher than that suggested by clinical examination, symptoms, and the spun haematocrit. This was found to be due to an interaction between the Coulter lysing agent and monomeric Ig...

By J. A. OWEN AND W. D. RIDER P ATIENTS WITH MYELOMATOSIS, whose electrophoretic serum protein patterns have contained a discrete component in the a2-globulin fraction, have been described on very few occasions; moreover, not all of the reported cases have been universally accepted as instances of “a2-type” myeloma protein. Indeed, Waldenstr#{246}m1’2 has questioned the existence of the “a2-typ...

introduction: infections by multidrug resistant mycobacterium tuberculosis (mdr-tb) is a major public health challenge. secretion of proteins by m. tuberculosis plays an important role in the pathogenesis of the bacterium. we compared the protein profiles of susceptible m. tuberculosis and mdr-tb isolates using proteomic analyses, namely two dimensional gel electrophoresis (2de) and mass spectr...

electrophoresis with the Hydrasys analyzer (Sebia), using Hydragel 15 HR gels (Sebia), missed a large IgM (12 g/L) monoclonal protein. The protein was detected by capillary electrophoresis and by the Beckman Paragon agarose electrophoresis system. The failure of the Hydrasys system to detect this monoclonal protein was related to the way sample application is performed with this semiautomated s...

The directed mutagenesis study of the Im7 protein of colicin E7 revealed that three residues, D31, D35, and E39, located in the loop 1 and helix 2 regions of the protein were critical for initiating the complex formation with its cognate colicin E7. Interestingly, the importance of these three critical residues in conferring specific immunity to its own colicin was exhibited in a hierarchical o...

Paraproteins have been estimated by both immunochemical methods and densitometric analysis of electrophoretic strips. Correlation between the results obtained by these two methods, although generally good for assessing trends, varied between patients.

Among different coronavirus genera, the receptor-binding S1 subunits of their spike proteins differ in primary, secondary, and tertiary structures. This study identified shared structural topologies (connectivity of secondary structural elements) in S1 domains of different coronavirus genera. The results suggest that coronavirus S1 subunits share a common evolutionary origin but have attained d...

In biochemistry laboratory, monoclonal immunoglobulins are frequently found. They can lead to analytical interferences which are difficult to anticipate. In this case reports, a monoclonal immunoglobulin M with cryoglobulin effect has been responsible of false result of protein electrophoresis because of the whole precipitation of the monoclonal immunoglobulin. This case report allows us to rem...

We presenta case ofmultiplemyeloma with high valuesfor total calcium.The case discussion focuses on the role of the laboratoryindiagnosisand monitoring ofmultiplemyeloma and on the causes ofthe associated hypercalcemia.We present evidence for calcium binding to the paraprotein as a cause for the “hypercalcemia” inthiscaseand we document a difference inthe resultsfortotalcalcium among various me...