No therapy is known to improve health-related quality of life (HRQoL) or dyspnoea in patients with idiopathic pulmonary fibrosis. The present study investigated longitudinal changes in HRQoL and dyspnoea and explored the effects of bosentan on these end-points during the Bosentan Use in Interstitial Lung Disease (BUILD)-1 trial. In total, 154 subjects received oral bosentan (n = 71) or placebo ...

Rhizosecretion of recombinant pharmaceuticals from in vitro hydroponic transgenic plant cultures is a simple, low cost, reproducible and controllable production method. Here, we demonstrate the application and adaptation of this manufacturing platform to a human antivitronectin IgG1 monoclonal antibody (mAb) called M12. The rationale for specific growth medium additives was established by pheno...

Bicifadine [DOV 220,075; (+/-)-1-(4-methylphenyl)-3-azabicyclo-[3.1.0]hexane HCl)] is a non-narcotic analgesic that has proven to be effective for the treatment of acute pain in clinical studies. The pharmacokinetics, disposition, and metabolism of bicifadine were determined in eight healthy adult male subjects following a single oral dose of 200 mg of [(14)C]bicifadine in solution. The maximum...

Two clones of the L5 myoblast line (M6 and the fusion-defective M12) were examined for the expression of myogenin, one of the regulatory genes involved in the regulation of differentiation to myofibers after treatment with 3-deazaadenosine, a metabolic inhibitor of methyl transfer reactions. Cultures treated with 3-deazaadenosine showed, using Northern blot hybridization, a conspicuous increase...

PURPOSE The aim of this study was to determine the influence of anatomical conditions on primary stability in the models simulating posterior maxilla. METHODS Polyurethane blocks were designed to simulate monocortical (M) and bicortical (B) conditions. Each condition had four subgroups measuring 3 mm (M3, B3), 5 mm (M5, B5), 8 mm (M8, B8), and 12 mm (M12, B12) in residual bone height (RBH). A...

T cell-APC conjugation as mediated by leukocyte function-associated antigen-1 (LFA-1)-intercellular adhesion molecule (ICAM)-1 binding is followed by formation of the supramolecular activation cluster (SMAC) at the immunological synapse. The intracellular processes that regulate SMAC formation and its influence on T cell function are important questions to be addressed. Here, using a mutational...

Keiichi Akasaka ([email protected]) Takahiro Tanaka ([email protected]) Nobutaka Kitamura ([email protected]) Shinya Ohkouchi ([email protected]) Ryushi Tazawa ([email protected]) Toshinori Takada ([email protected]) Etsuro Yamaguchi ([email protected]) Toshio Ichiwata ([email protected]) Masaki Hirose ([email protected]) To...

Diosbulbin B (DB), a major constituent of the furano-norditerpenes in Dioscorea bulbifera Linn, exhibits potential antineoplasmic activity and hepatotoxicity. The metabolism and reactive metabolites of DB in vitro (with human and animal liver microsomes) and in vivo in rats were investigated. The human enzymes involved in DB metabolism were identified. DB was first catalyzed into reactive metab...