نتایج جستجو برای: mannose binding lectin gene

تعداد نتایج: 1446737  

2012
Harshvardan Khare Vivek Ratnaparkhi Sonali Chavan Valadi Jayraman

Mannose is an abundant cell surface monosaccharide and has an important role in many biochemical processes. It binds to a great diversity of receptor proteins. In this study we have employed Random Forest for prediction of mannose binding sites. Mannosebinding site is taken to be a sphere around the centroid of the ligand and the sphere is subdivided into different layers and atom wise and resi...

Journal: :Acta crystallographica. Section F, Structural biology and crystallization communications 2010
Mads Gabrielsen Puteri Shafinaz Abdul-Rahman Neil W Isaacs Onn Haji Hashim Richard J Cogdell

Mannose-binding lectin from champedak (Artocarpus integer) is a homotetramer with a single-monomer molecular weight of 16 800 Da. Previous work has shown it to bind IgE and IgM, as well as being a mitogen of T cells in humans. Champedak mannose-binding lectin has successfully been used to detect altered glycosylation states of serum proteins. The protein was crystallized at 293 K in space group...

Journal: :Glycobiology 2007
Aleksandr A Bulgakov Marina G Eliseikina Irina Yu Petrova Evgeny L Nazarenko Svetlana N Kovalchuk Valery B Kozhemyako Valery A Rasskazov

To elucidate the origin and evolution of mannan-binding lectins (MBL), a new C-type lectin (CTL) specific for high-mannose glycans (MBL-AJ) was isolated from the coelomic plasma of the holothurian Apostichopus japonicus. MBL-AJ has oligomeric forms with identical 17-kDa subunits on SDS-PAGE. Among natural ligands, lectin hemagglutination activity was competitively inhibited by extracellular low...

Journal: :Journal of bacteriology 1998
O Kobayashi N Hayashi R Kuroki H Sone

Yeast flocculation is a phenomenon which is believed to result from an interaction between a lectin-like protein and a mannose chain located on the yeast cell surface. The FLO1 gene, which encodes a cell wall protein, is considered to play an important role in yeast flocculation, which is inhibited by mannose but not by glucose (mannose-specific flocculation). A new homologue of FLO1, named Lg-...

Journal: :BMB reports 2008
Bo Liu Xiao-Chao Xu Yan Cheng Jian Huang Yan-Hong Liu Zhen Liu Ming-Wei Min He-Jiao Bian Jing Chen Jin-Ku Bao

Polygonatum cyrtonema Lectin (PCL), which is classified as a monocot mannose-binding lectin, has received great regards for its uniquely biological activities and potentially medical applications in cancer cells. This paper was initially aimed to study apoptosis of PCL on Hela cells. Thus, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method was carried out. Through obser...

Journal: :Journal of virology 2009
Zhi-Ying Zhao Zhi-Xin Yin Xiao-Peng Xu Shao-Ping Weng Xia-Yu Rao Zong-Xian Dai Yong-Wen Luo Gan Yang Zong-Sheng Li Hao-Ji Guan Se-Dong Li Siu-Ming Chan Xiao-Qiang Yu Jian-Guo He

C-type lectins play key roles in pathogen recognition, innate immunity, and cell-cell interactions. Here, we report a new C-type lectin (C-type lectin 1) from the shrimp Litopenaeus vannamei (LvCTL1), which has activity against the white spot syndrome virus (WSSV). LvCTL1 is a 156-residue polypeptide containing a C-type carbohydrate recognition domain with an EPN (Glu(99)-Pro(100)-Asn(101)) mot...

Journal: :Cell 2010
Isolde Le Trong Pavel Aprikian Brian A. Kidd Manu Forero-Shelton Veronika Tchesnokova Ponni Rajagopal Victoria Rodriguez Gianluca Interlandi Rachel Klevit Viola Vogel Ronald E. Stenkamp Evgeni V. Sokurenko Wendy E. Thomas

The Escherichia coli fimbrial adhesive protein, FimH, mediates shear-dependent binding to mannosylated surfaces via force-enhanced allosteric catch bonds, but the underlying structural mechanism was previously unknown. Here we present the crystal structure of FimH incorporated into the multiprotein fimbrial tip, where the anchoring (pilin) domain of FimH interacts with the mannose-binding (lect...

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