Myoglobin (Mb) interaction with the outer mitochondrial membrane (OMM) promotes oxygen (O2) release. However, comprehensive molecular details on specific contact regions of OMM oxygenated (oxy-) and deoxygenated (deoxy-)Mb are missing. We used dynamics (MD) simulations to explore oxy- deoxy-Mb lipids in two lipid compositions: (a) a typical whole average, (b) specifically cardiolipin-enriched c...

ndrew G. C. Sutton, MA, MB, MRCP,* Philip G. Campbell, MB, MRCP,* ichard Graham, MB, MRCP,* Dallas J. A. Price, MB, MRCP,* Janine C. Gray, BSC(HONS), PHD,† ver D. Grech, MD, MRCP, FACC,* James A. Hall, MA, MD, FRCP,* lun A. Harcombe, MD, MRCP,* Robert A. Wright, MD, FRCP,* oger H. Smith, BSC(HONS), MB, FRCP,‡ Jerry J. Murphy, MB, BS, DM, FRCP,§ nanthaiah Shyam-Sundar, MB, BS, MD, DM, FRCP,‡ Mic...

The present study aimed to investigate the roles of the vascular endothelial cell growth factor (VEGF) and micro (mi)RNA-210 in the metastasis of primary medulloblastoma (MB) tumors. A total of 86 adult patients diagnosed with cerebellar MB were enrolled in the present study, of which 11 patients had metastatic MB in the subarachnoid space. The following samples were collected: MB primary tumor...

Incidence, Event Rates, and Early Outcome of Stroke in Dublin, Ireland: The North Dublin Population Stroke Study Peter J. Kelly, MD; Gloria Crispino, PhD; Orla Sheehan, MB; Lisa Kelly, MB; Michael Marnane, MB; Aine Merwick, MB; Niamh Hannon, MB; Danielle Ní Chróinín, MB; Elizabeth Callaly, MB; Dawn Harris, BSc; Gillian Horgan, BSc; Emma B. Williams, PhD; Joseph Duggan, MD; Lorraine Kyne, MD; Pa...

Background:Organophosphates are commercially available agrochemicals for pest control, but may be abused for deliberate self-poisoning. We report a case of suicidal ingestion of dimethoate, a moderately toxic organophosphate insecticide, which can cause refractory hemodynamic instability, and the successful use of methylene blue (MB) for counteracting this complication. Case Presentation: An u...

Although drug resistance is often observed in metastatic recurrence of breast cancer, little is known about the intrinsic drug resistance in such metastases. In the present study, we found, for the first time, that MDA-MB-231BR, a brain metastatic variant of a human breast cancer cell line, was refractory to treatment with 5-fluorouracil (5-FU) even without chronic drug exposure, compared to it...

BACKGROUND Heart rate (HR) recovery (HRR) reflects autonomic activity and predicts cardiovascular events. The aim of this study was to assess HRR in patients with myocardial bridge (MB). METHODS Medical recordings of 93 patients with MB and appropriate age, compared to 78 sex-matched healthy subjects were analyzed. MB was diagnosed via coronary computed tomography angiography after a positive...

A thiazine dye reductase has been described in endothelial cells that reduces methylene blue (MB), allowing its uptake into cells. Because a different mechanism of MB uptake in human erythrocytes has been proposed, we measured MB uptake and reduction in this cell type. Oxidized MB (MB(+)) stimulated reduction of extracellular ferricyanide in a time- and concentration-dependent manner, reflectin...

Myoglobin (Mb) is an oxygen-binding muscular hemeprotein regulated via Ca(2+)-signaling pathways involving calcineurin (CN), with Mb increases attributed to hypoxia, exercise, and nitric oxide. Here, we show a link between lipid supplementation and increased Mb in skeletal muscle. C2C12 cells were cultured in normoxia or hypoxia with glucose or 5% lipid. Mb assays revealed that lipid cohorts ha...