The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of medicinally important plant Taraxacum officinale in its aqueous and methanol extract of root, stem and flower. Saponins, flavonoids, alkaloids, phenols were highly concentrated in the stem, root and flower, with the higher concentration of flavonoids in the flower extracts. Phenols an...

Biodiesel, known as fatty acid methyl ester (FAME), was produced from crude cottonseed oil (triglycerides) by transesterification with methanol in the presence of sodium hydroxide. This process was optimized by applying factorial design and response surface methodology (RSM) with SAS and PSIPLOT programs. A second-order mathematical model was obtained to predict the yield as a function of metha...

Optimization of protein production from methanol-induced Pichia pastoris cultures is necessary to ensure high productivity rates and high yields of recombinant proteins. We investigated the effects of temperature and different linear or exponential methanol-feeding rates on the production of recombinant Fusarium graminearum galactose oxidase (EC 1.1.3.9) in a P. pastoris Mut(+) strain, under re...

This study is based on optimizing the experimental conditions of biodiesel production by base-catalyzed transesterification using waste cooking oil (WCO). In this study, the key parameters varied were methanol (20, 25, 30, 35, and 40%), sodium hydroxide (0.4, 0.6, 0.8, 0.9 and 1.0g), reaction time (40, 60, 90, 100 and 120 minutes) and reaction temperature (50, 52, 55, 58, and 60 C). Maximum bio...

Cultures from the unicellular green alga Scenedesmus obliquus biodegrade the toxic p-cresol (4-methylphenol) and use it as alternative carbon/energy source. The biodegradation procedure of p-cresol seems to be a two-step process. HPLC analyses indicate that the split of the methyl group (first step) that is possibly converted to methanol (increased methanol concentration in the growth medium), ...

We evaluated the antioxidant activity and tyrosinase inhibitory effects of Pleurotus ostreatus fruiting bodies extracted with acetone, methanol, and hot water. The antioxidant activities were tested against β-carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, and ferrous chelating ability. Furthermore, phenolic acid and flavonoid contents wer...

We have developed a yeast Pichia pastoris system for the high-level expression of recombinant Rhizopus oryzae lipase (ROL), which is a potentially effective catalyst in the solvent-free production of biodiesel fuel. In the glycerol fed-batch phase, the combination of the dissolvedoxygen-stat and gradient-control glycerol feeding strategies resulted in a higher cell biomass in the P. pastoris cu...

Background & Aim:Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of existing drugs. The present study aimed to compare antimicrobial effects of methanol extract of Francoeuria Undul...

Pichia pastoris is a methylotrophic yeast used as an efficient expression system for heterologous protein production as compared to other expression systems. Considering that every cell must respond to environmental changes to survive and differentiate, determination of endogenous protein related to heat stress responses and hypoxia, it would necessary to establish the temperature and methanol ...

The response of the Intoxilyzer 8000C (version approved for evidentiary breath alcohol testing in Canada) to volatile solvents in vitro is described. Acetone, isopropanol, and methanol were prepared as aqueous solutions or dilutions of standard alcohol solution (SAS; 1.21 mg ethanol/mL) to generate apparent blood ethanol concentrations (aBEC) of 50 or 80 mg/dL. Solvent concentrations examined w...