Staphylococcus pseudintermedius is the most common microorganism isolated from canine pyoderma and postoperative wound infections. The prevalence of methicillin-resistant S. pseudintermedius (MRSP) has increased, and recently, isolates that are resistant not only to methicillin but also to other classes of antibiotic drugs, including aminoglycosides, have become common. A total of 422 S. pseudi...

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methi...

Detection of methicillin resistance in Staphylococcus aureus is a challenge, especially low-level resistance, which is often misdiagnosed. The aim of this study was to compare the diagnostic accuracies of the automated Vitek 2 system and disk diffusion tests, using cefoxitin and moxalactam, for the detection of methicillin resistance in S. aureus strains. Four sets of genotypically diverse isol...

The anti-staphylococcal activity of ABT-773, a new member of the ketolides, was examined and compared with those of erythromycin and clarithromycin using the agar dilution method. A total of 336 well-defined clinical isolates of staphylococci, including a large number of clonally different methicillin-resistant strains with defined macrolide-lincosamide-streptogramin B (MLS(B)) resistance pheno...

Infected non-union of long bones is a challenge to manage. It has a high morbidity and mortality. Treatment is very demanding and has a significant complication rate. Methicillin-resistant infected non-union has a higher morbidity and mortality compared to Methicillin-sensitive Staphylococcus aureus infection. Approximately half of all Staphylococci isolated in infected orthopaedic operations a...

28 new pyrrolidine types of compounds as analogues for natural polyhydroxy alkaloids of codonopsinine were evaluated for their anti-MRSA activity using MIC and MBC value determination assay against a panel of S. aureus isolates. One pyrrolidine compound, MFM 501, exhibited good inhibitory activity with MIC value of 15.6 to 31.3 μg/mL against 55 S. aureus isolates (43 MRSA and 12 MSSA isolates)....

OBJECTIVES To analyse the possible relationship between consumption of old and new MRSA-active antibiotics and burden of MRSA in acute care hospitals in Catalonia during the period 2007-12. METHODS Fifty-four hospitals participating in the VINCat Programme were included. Proportion of MRSA (resistant isolates of Staphylococcus aureus per 100 isolates of S. aureus tested), incidence of new cas...

Methods From April 2008 to March 2010, at Imperial College Healthcare NHS Trust (ICHNT), electronic microbiological records of patients with SAB were linked with patient administration data to determine if the S. aureus was meticillin-sensitive or resistant, and if infection was CA or HA. SAB within 2 days of admission was defined as CA, 2 days or more after admission as HA and a new episode if...

Staphylococci are the leading cause of nosocomial blood stream infections. Fast and accurate identification of staphylococci and confirmation of their methicillin resistance are crucial for immediate treatment with effective antibiotics. A multiplex real-time PCR assay that targets mecA, femA specific for S. aureus, femA specific for S. epidermidis, 16S rRNA for universal bacteria, and 16S rRNA...

PURPOSE OF REVIEW The scourge of community-acquired methicillin-resistant Staphylococcus aureus in pediatrics continues unabated. This review provides information on changes in epidemiology, therapeutic considerations, and measures to control the epidemic. RECENT FINDINGS The epidemiology and clinical manifestations of methicillin-resistant S. aureus have undergone important changes that pose...