Three lines of evidence indicated that methionine sulfoxide is transported by the high-affinity methionine and glutamine transport systems in Salmonella typhimurium. First, methionine-requiring strains (metE) which have mutations affecting both of these transport systems (metP glnP) were unable to use methionine sulfoxide as a source of methionine. These strains could still grow on L-methionine...

the 440 fertile eggs, in a completely randomized design were divided into 11 treatments with four replicatesand 10 eggs per each replicate. treatments including: (t1; control; without injection), (t2; injected with 0.3ml saline 9.0%; sham control), (t3, t4 and t5) 25, 75, 125 ppm fe-nano, (t6, t7 and t8) 50, 100, 150 ppmfe-nano-alimet chelate, (t9, t10 and t11) 50, 100, 150 ppm fe-aliment chela...

The objectives of this study were to evaluate ruminal degradation and intestinal digestion of two ruminally protected methionine (RPM) products and to assess the potential use of changes in plasma methionine concentrations as an indication of methionine availability to the animal. Ruminal degradation of the protected methionine was assessed using the in situ technique. The intestinal availabili...

PURPOSE Many neoplasms are vulnerable to methionine deficiency by mechanisms that are poorly understood. Because gene profiling studies have revealed that methionine depletion increases TNF-related apoptosis-inducing ligand receptor-2 (TRAIL-R2) mRNA, we postulated that methionine stress sensitizes breast cancer cells to proapoptotic TRAIL-R2 agonists. EXPERIMENTAL DESIGN Human triple (ER/PR/...

Methionine has been shown to increase plasma cholesterol in animals. In the present study, mechanisms were investigated by which methionine could alter cholesterol metabolism. In the first experiment, forty growing rats were fed four casein-based diets differing in methionine content (2.6, 3.5, 4.5 or 6.0 g/kg) for 14 d. In the second experiment, isolated rat hepatocytes were incubated in media...

The Bacillus subtilis yusCBA operon, which encodes an ABC-type transporter, contains an S-box motif in its promoter region. We showed that the expression of these genes is repressed via the S-box system when methionine is available. The YusCB proteins are involved in the transport of both d- and l-methionine but also methionine sulfoxide. A yusCB mutant is unable to grow in the presence of 5 mi...

The death of a control subject after an oral load of methionine for a study of the possible relationship between homocysteine and Alzheimer’s disease is reported. The subject developed postload plasma concentrations of methionine far beyond those reported previously in humans given the usual oral loading dose of methionine (100 mg/kg body wt). Her preload plasma metabolite values rule out known...