ABSTRACT Saturated salt solutions produce environments with specific relative humidities used in several applications, such as determining water sorption isotherms of foods. However, the preparation saturated requires knowledge solubility data. Thus, objective this work was to determine 14 salts: lithium bromide (LiBr), chloride (LiCl), iodide (LiI), magnesium (MgCl2), sodium (NaI), potassium c...

There is an inevitable difference between direct and indirect contact of metals with eutectic chloride melts defined by the gas phase impurity content, here, humidity oxygen in argon. The impurities-to-salt ratio will always be higher allowing for a more aggressive attack on metal components. This effect has been studied decommissioned container from experimental high-temperature setup, which n...

The present experimental investigation was carried out in order to explore the possible thermodynamic and interactions studies of D(+) fructose with mixed solvent of aqueous alkali metal halides at different molalities and constant temperature. Experimental values of densities and ultrasonic velocities were carried out of the ternary mixture solution D(+)fructose with aqueous NaCl ,KCl , MgCl2 ...

G-Actin was extracted from the acetone powder of the dark muscle of mackerel Pneumato phorus japonicus japonicus, and purified by a method which essentially consisted of polymerization in 0.1M KCl containing 0.1mm MgCl2, treatment with 0.6M KCI, and Sephadex G-200 gel filtration . Sedimentation constant (s020,w) of the dark muscle actin was 2.9S. The molecular weight was determined to be 42,000...

The nucleosome is the first level of genome organization and regulation in eukaryotes where negatively charged DNA is wrapped around largely positively charged histone proteins. Interaction between nucleosomes is dominated by electrostatics at long range and guided by specific contacts at short range, particularly involving their flexible histone tails. We have thus quantified how internucleoso...

For more than a century, the Hofmeister Series has been an open problem in biophysics and colloid science. In this experiment, a standard viscometer has been used to test a section of the Series by comparing the viscosities of simulated egg white solutions after the addition of different salts (KCl,NaCl,MgCl2, CaCl2, LiCl and LiBr). Considering the obtained data, we have that the trend of the L...

Dextran glucosidase from Streptococcus mutans (SmDG), which belongs to glycoside hydrolase family 13 (GH13), hydrolyzes the non-reducing terminal glucosidic linkage of isomaltooligosaccharides and dextran. Thermal deactivation of SmDG did not follow the single exponential decay but rather the two-step irreversible deactivation model, which involves an active intermediate having 39% specific act...

The influence of NaCl, KCl, CaCl2, and MgCl2 on the binding of prolactin (PRL) to its receptor was investigated. The salts were dissolved in a metallic ion-free binding buffer and had biphasic effects on changes in the association rate constant (k+1) of PRL binding, depending on their concentrations: there was an increase in the k+1 at lower concentrations and a decrease at higher concentration...

The present study is concerned with the improvement of Bacillus licheniformis strain GCB30 for alpha amylase production in 250 ml Erlenmeyer flasks. Chemical mutation using ethyl methanesulfonate (EMS 50-300 μ/ml) was undertaken for 10-60 min. Twenty eight isolates were selected on the basis of clear zones of starch hydrolysis. Only one isolate designated as B. licheniformis EMS-200 gave 102.78...

plants and soil microbes are the main sources of most enzymes available in the environment. it is, therefore, anticipated that any change in the growth of soil biota in response to salinity, may reduce the synthesis and production of extra- and intra- cellular enzymes. as a result, a reduction in the growth of plants and the activity of soil microbes in saline environments may lessen the level ...