For broth microdilution susceptibility tests of Francisella tularensis, Mueller-Hinton broth with 2% Isovitalex is recommended. Using that medium, we studied three standard control strains tested with nine antimicrobial agents potentially efficacious for treating tularemia. An eight-laboratory collaborative study generated the data needed to propose appropriate MIC control limits.

The stability of eight antimicrobial agents in WILKENS-CHALGREN broth was evaluated. The activities of only carbenicillin and benzyl penicillin were significantly decreased after storage for eight weeks at--20 degrees C. Anaerobic reduction of the susceptibility testing broth prior to inoculation was found to be unnecessary.

EUCAST expert rules have been developed to assist clinical microbiologists and describe actions to be taken in response to specific antimicrobial susceptibility test results. They include recommendations on reporting, such as inferring susceptibility to other agents from results with one, suppression of results that may be inappropriate, and editing of results from susceptible to intermediate o...

The fluconazole MIC distributions for Candida glabrata from testing 34 different clinical isolates and performing 51 tests on a single isolate mirrored each other. Since what is perceived as biological variation in isolates without resistance mechanisms is mainly methodological variation, breakpoints which divide this distribution not only lack a sound biological basis but also result in poor r...

Theselectionof chemotherapy inaquatic animalmedicine is not as straightforward as one might believe. A multitude of factorscan impacteffectiveness insitu. Someof these factors include the pathogen(s) present and their antimicrobial susceptibility, site(s) of infection, timing of treatment, host health/disease status, dose and regimen, water salinity, and water temperature. This article will foc...

BACKGROUND Asymptomatic bacteriuria in pregnancy is associated with potential urinary and obstetric complications. However the prevalence aetiology and antimicrobial sensitivity patterns of asymptomatic bacteriurea among women attending ante-natal care in our Hospital is not known. OBJECTIVE To determine the prevalence and identify the aetiological agents associated with asymptomatic bacteriu...

Abstract-Elevated metal concentrations in soils can disturb the soil ecosystem; thus, researchers strive to identify the most sensitive assay for detection of the early signs of toxicity. The purpose of the present study was to compare eight different ecotoxicological endpoints on the same set of metal-contaminated soils that were collected from seven series of soils sampled during field trials...

The AutoSCAN-3 (American MicroScan, Mahwah, N.J.) is an instrument capable of automated reading of commercially available microdilution trays for identification and quantitative susceptibility testing of rapidly growing bacteria. This study compared the results of visual and automated reading of microdilution trays for determination and interpretation of minimum inhibitory concentrations of 471...

The speed at which most countries with high burdens of multidrug-resistant tuberculosis (MDRTB) have scaled up their capacity to diagnose and treat individuals with these forms of TB has failed to keep pace with the problem. Limited availability of drug susceptibility testing, high costs and inefficiencies in the supply of second-line drugs, and inadequate capacity for the management of patient...

Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is simple, and could be used to determine the antifungal susceptibility of dermato...