A radioisotopic assay is reported that 4 hr and can be run in unlimited quanaccurately measures an almost identical tity. This raclioassay should simplify diserum folate to that measured by Lactoagnosis of folate deficiency and be parbacillus casei and can separate low, ticularly useful in patients receiving borderline, and normal folate levels. This antibiotics and folate antagonists, where as...

background mycoplasma synoviae is an important avian pathogen which can cause both respiratory disease and synovial joint inflammation (synovitis) in poultry. mycoplasmas spp. may cause the respiratory system infection in ostriches with symptoms such as inflammation of the nose, trachea and also damages of lungs. objectives the current study aimed to use the m. synoviae specific polymerase chai...

We describe here a simple, fast, and reliable bioassay method for therapeutic drug monitoring of voriconazole. Fifty-eight clinical and external quality control samples were evaluated with this microbiological assay, and results were compared with those obtained with a previously validated chromatographic method. A good correlation between both assays was observed. This particular microbiologic...

Both fluorometric and microbiological measurements of urinary riboflavin excretion have served as a basis for evaluation of nutritional status, detection of deficiency states, measurement of physiological availability, and determination of daily requirements. Fluorometric assay methods have commonly followed three basic procedures; namely, (a) that of Najjar (l), based on the extraction of ribo...

Owing to the higher serum cobalamin results that are obtained by R-binder radioisotopic dilution assay compared to microbiological assays (E. gracilis and L. leichmannii) it was suggested that serum contained a cobamide(s) that could not be detected by the more specific microbiological assays and that a much less specific test organism, which responds to most naturally occurring cobamides, such...

The emergence of a strain of Providencia stuartii resistant to multiple antibiotics with the exception of amikacin provided a test organism for a microbiological assay for this new semisynthetic aminoglycosidic aminocyclitol. Results can be read at 4 h and are specific for amikacin. The resistance of P. stuartii to all currently used antibiotics allows the utilization of this technique in the p...

BACKGROUND/METHODS We compared the diagnostic yield of a real time polymerase chain reaction (PCR) assay in cerebrospinal fluid (CSF) samples with conventional microbiological techniques for the diagnosis of neurobrucellosis. Following amplification of a 223 bp sequence specific for Brucella genus, melting curve analysis was performed to verify the specificity of the PCR products. RESULTS All...

Background/methods: We compared the diagnostic yield of a real time polymerase chain reaction (PCR) assay in cerebrospinal fluid (CSF) samples with conventional microbiological techniques for the diagnosis of neurobrucellosis. Following amplification of a 223 bp sequence specific for Brucella genus, melting curve analysis was performed to verify the specificity of the PCR products. Results: All...

BACKGROUND Water and High Purity Water (HPW) distribution systems can be contaminated with human pathogenic microorganisms. This biocontamination may pose a risk to human health as HPW is commonly used in the industrial, pharmaceutical and clinical sectors. Currently, routine microbiological testing of HPW is performed using slow and labour intensive traditional microbiological based techniques...