Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature e...

The study was carried out to establish an EV71 virus culture system on Vero cells in a shake (Wave bioreactor) incorporating microcarriers for the production of hand, foot and mouth (HFM) vaccine caused by virus. results investigation 2 types (Cytodex 1 Cytodex 3), showed that gave better efficiency when culturing suspension conditions. combination with Wave bioreactor cell over 3x106cells/ml. ...