radiography is one of the most valuable diagnostic tools used in comprehensive dental care. although there is no safe level of radiation exposure, the possible risk associated with exposure to radiation, must be elucidated. to date, a variety of assays have been proposed to assess the mutagenic potential of genotoxicants; however, these methods are typically laborious and time consuming. the ai...

Occupational exposure to anaesthetic gases is associated with various adverse health effects. Genetic material is a sensitive target of numerous harmful agents. The aim of this study was to examine whether chromosomal damage could serve to indicate exposure to anaesthetics. Twenty-eight anaesthesiologists, 16 technicians, and 32 control subjects were examined for chromosome aberrations and micr...

The macronucleus of the protozoan Oxytricha fallax is generated from a micronucleus following conjugation. While the micronucleus contains high molecular weight DNA, the macronucleus contains only short linear DNA molecules which all end in the same 20 bp inverted terminal repeat (Ma-ITR). The Ma-ITR was radioactively labeled and purified for use as a probe in hybridizations to micronuclear and...

Rivers in the KPK Peshawar region of Pak are subjected to a variety anthropogenic impacts, Agricultural operations, industry, and urban garbage all examples. The purpose goal this research was find out degree water contamination by showing testing that includes situ bio-monitoring constituents on Geno-toxicity local fish species. Three places were tested for using micronucleus test. At sample l...

Background: Genomic instability is one of primary causes for malignant cell transformation. In this study induced genomic instability expressed as micronuclei in breast cancer (BC) patients with different stages of the disease compared with their unaffected first degree relatives (FDR) and normal unrelated controls was investigated. Materials and Methods: The background and net micronucleus fre...

the aim of this study was the evaluation of acute genotoxic effect of effluent water of thermo-power plant by means of tradescantia root tips micronucleus test (mn), mitotic index and cell aberrations.   tradescantia , was experimentally treated (for 24 h), with effluent water of thermo-power plant in different dilution ratios (negative control – distilled water; primary untreated effluent wate...

We have used viscoelastometry in an attempt to understand the physical organization of genetic material in Tetrahymena nuclei. The micronucleus or germ line nucleus is diploid. It divides mitotically during vegetative growth, and five pairs of chromosomes are seen in meiosis. The macronucleus, or somatic nucleus, is approximately 45-ploid, divides amitotically, and has no visible chromosomes at...

We evaluated the antimutagenic effects of 10 kinds of bioactive phytochemicals and some phytochemical combinations against methotrexate (MTX)-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/pSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other ...

The increased use of biostimulants in conventional agriculture and organic farming requires the implementation rapid tests to determine their effectiveness enhancing plant growth protection against abiotic stresses. However, innocuity health has rarely been demonstrated. We used Vicia faba Micronucleus Assay, as described by standard AFNOR EN ISO 29200(2020-05) reveal biostimulant, genotoxic cy...

Chios mastic gum, a plant-derived product obtained by the Mediterranean bush Pistacia lentiscus (L.) var. chia (Duham), has generated considerable interest because of its antimicrobial, anticancer, antioxidant and other beneficial properties. Its aqueous extract, called Chios mastic water (CMW), contains the authentic mastic scent and all the water soluble components of mastic. In the present s...