Aluminium (Al) toxicity is an important limitation to barley (Hordeum vulgare L.) on acid soil. Al-resistant cultivars of barley detoxify Al externally by secreting citrate from the roots. To link the genetics and physiology of Al resistance in barley, genes controlling Al resistance and Al-activated secretion of citrate were mapped. An analysis of Al-induced root growth inhibition from 100 F2 ...

Accurately estimating inbreeding is important because inbreeding reduces fitness and production traits in populations. We analyzed information from pedigrees and from microsatellite markers to estimate inbreeding in a line of Japanese quail derived from a randombred line (QO) and maintained for 17 generations by pedigreed matings of brothers to groups of sisters. Pedigree data were used to calc...

background: indigenous chickens could serve as precious genetic resources that should be considered in conservation and breeding programs. the major histocompatibility complex (mhc) has a strong association to disease resistance/susceptibility, production and reproduction traits in chicken. therefore, identifying its polymorphism in populations under selective breeding could be used for selecti...

A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.0370 to 0.8927. Linkage equilibrium was ob...

A major susceptibility locus for leprosy has recently been mapped on chromosome 10 (10p13) by genome-wide linkage analysis. Microsatellite markers from this genome screen that showed suggestive evidence of linkage to leprosy were evaluated in an additional 140 families with affected sib pairs. A second region of linkage has thus been identified on chromosome 20 (20p12). The peak of linkage lies...

UNLABELLED PREMISE OF THE STUDY To elucidate the population genetics and phylogeography of Scrophularia incisa, microsatellite primers were developed. We also applied these microsatellite markers to its closely related species S. dentata and S. kiriloviana. • METHODS AND RESULTS Using the compound microsatellite marker technique, 12 microsatellite primers were identified in S. incisa. The ...

The aim of this study was to evaluate the Bali cattle genetic diversity and hybrid cattle using microsatellite markers. Blood samples (n=192) were collected from various cattle breed, i.e. Bali (n=96), Madura (n=48), and Peranakan Ongole (PO) Kebumen (n=48). The microsatellite locus used for assessing the genetic variation was INRA035, ILSTS006, ETH225, and HEL9, while the genetic profile was d...

Microsatellite sequences are important markers for population genetics studies. In the past, the development of adequate microsatellite primers has been cumbersome. However with the advent of next-generation sequencing technologies, marker identification in genomes of non-model species has been greatly simplified. Here we describe microsatellite discovery on a Pacific Biosciences single molecul...

We focus on the development of microsatellite loci isolated from two scleractinian coral species (Montastraea cavernosa and Porites astreoides). To analyze the genetic structure of scleractinian coral populations, it is essential to identify a genetic marker that can evaluate individual or population-level differences (Shearer and Coffroth 2004). Optimal PCR conditions are essential when develo...