background: nowadays, microencapsulation of probiotic bacteria is the newest method for increasing the survival ability of probiotics in food products. the lactobacillus acidophilus as a probiotic bacterium has more beneficial effects and the low survival of this bacterium under food processing conditions and adverse environment have been studied by many researchers. objectives: in this study, ...

Plant-mediated synthesis of metal oxide nanoparticles is a promising alternative to the traditional method of physical and chemical synthesis. In this paper, we report the green synthesis of zinc oxide nanoparticles (ZnONPs) by a biological method. During the study, Zinc oxide nanoparticles were synthesized by Allium sativum skin (garlic skin) extract. Formation of zinc oxide nanoparticles has ...

A quantitative comparison of two restoration methods as applied to confocal microscopy, Proc. 1. Introduction The main contribution of the confocal microscope to microscopy is that it provides a practical method to obtain microscopic volume images. Although a confocal microscope is a true volume imager, its imaging properties give rise to a blurring phenomenon similar to that of a conventional ...

nowadays, atomic force microscope is considered as a useful tool in the determination of intermolecular forces and surface topography with the resolution of nanometers. in this kind of microscope, micro cantilever is considered as the heart of the microscope and is used as a measuring tool.  this paper is aimed towards investigating the behavior of a piezoelectric micro cantilever with a triang...

Recently, confocal microscope has become a routine technique and indispensable tool for cell biological studies and molecular investigations. The light emitted from the point out-of-focus is blocked by the pinhole and can not reach the detector, which is one of the critical features of the confocal microscope. In present studies, the probes acridine orange (AO) and rhodamine-123 were used to re...

The analysis of electron micrographs by optical diffraction was introduced recently by Klug & Berger (1964). Their experiments were conducted with a special diffractometer designed for use with diffracting masks up to several inches in diameter. A method is described here for using a conventional light microscope as an optical diffractometer which can accept masks up to 5 mm in diameter. A 100-...

Microscope has been widely used to observe microscopic objects. The types of microscope are varied according to its function. The conventional optical microscope only provides intensity information of an object and it has some difficulties to provide information about the real shape of the object and the projection of the object in different depth and perspective. In addition, optical microscop...

Nano-resolution fluorescence electron microscopy (nano-fEM) pinpoints the location of individual proteins in electron micrographs. Plastic sections are first imaged using a super-resolution fluorescence microscope and then imaged on an electron microscope. The two images are superimposed to correlate the position of labeled proteins relative to subcellular structures. Here, we describe the meth...

In this work, we propose a structured illumination (SI) method based on a twophoton excitation (TPE) scanning laser beam. Advantages of TPE methods include optical sectioning, low photo-toxicity, and robustness in the face of sample induced scattering. We designed a novel multi-spot point spread function (PSF) for a fast, two-photon scanning SIM microscope. Our multi-spot PSF is generated with ...

The author have developed a new SCC test method that enables the super Kelvin force microscope (SKFM) and the Kelvin force microscope (KFM) observations. By using this test device, the crack tip deformation and surface potential distribution were observed on SUS310S stainless steel by SKFM and KFM. At the same time, the distribution of hydrogen was examined by the Ag decoration method with the ...