This study reevaluates the putative advantages of microwave-assisted tryptic digests compared to conventionally heated protocols performed at the same temperature. An initial investigation of enzyme stability in a temperature range of 37-80 °C demonstrated that trypsin activity declines sharply at temperatures above 60 °C, regardless if microwave dielectric heating or conventional heating is em...

Kentsel yerleşim alanlarındaki nüfus artışı, belediye atıksu arıtma tesislerinde (AAT) biyolojik çamur üretiminde artışa neden olmaktadır. Yüksek bertaraf maliyeti ve yasal kısıtlama, AAT’nde yönetimi konusunda karşılaşılan en önemli sorunlardır. Son yıllarda, AAT'nde atık üretimini azaltmak anaerobik çürütme ünitesinde üretilen biyogaz hacmini artırmak için daha çevreci ekonomik yöntemler geli...

A microwave-assisted digestion method for the determination of cadmium (Cd) and lead (Pb) by inductively coupled plasma mass spectrometry (ICP-MS) was optimised on certified reference material (CRM) (bovine liver, BCR-185R) and wolf liver samples. Different factors influencing digestion efficiency (temperature, time, composition of the digestion mixture, sample mass) were tested. Validation inc...

water-pollution control analysis. It is closely related to the organic contamination level of a water or wastewater sample. The Cr-COD method is used as the standard in USA and Taiwan. Compared to Japanese Mn-COD method, it has a higher detection limit and severe chloride ion interference because of the stronger oxidant and the longer digestion time. In order to reduce the chloride-ion interfer...

OBJECTIVE To measure the concentrations of the trace elements lead and cadmium in human placenta and in maternal and neonatal (cord) blood. To assess the influence of the strongly polluted environment on the content of metals in tissues and on the permeability of placenta to cadmium and lead. Various methods of mineralisation were tested before analysis. METHODS Graphite furnace atomic absorp...

Microwave-assisted sample preparation using diluted nitric acid solutions is an alternative procedure for digesting organic samples. The efficiency of this procedure depends on the chemical properties of the samples and in this work it was evaluated by the determination of crude protein amount, fat and original carbon. Soybeans grains, bovine blood, bovine muscle and bovine viscera were digeste...