Various types of stem cell lines have been derived from preimplantation or postimplantation mouse embryos: embryonic stem cell lines, epiblast stem cell lines, and trophoblast stem cell lines. It is not known if extraembryonic endoderm stem (XEN) cell lines can be derived from postimplantation mouse embryos. Here, we report the derivation of 77 XEN cell lines from 85 postimplantation embryos at...

Basic fibroblast growth factor (FGF2)-responsive definitive neural stem cells first appear in embryonic day 8.5 (E8.5) mouse embryos, but not in earlier embryos, although neural tissue exists at E7.5. Here, we demonstrate that leukemia inhibitory factor-dependent (but not FGF2-dependent) sphere-forming cells are present in the earlier (E5.5-E7.5) mouse embryo. The resultant clonal sphere cells ...

neurotrauma is one of the main medical problems that affect considerable number of population especially young persons in all countries. neurotrauma not only is a serious threat for life but also creates various disabilities and consequently imposes large costs to society. following central nervous system (cns) injury, tissue damage occurs via primary and secondary mechanisms. despite many atte...

Knelangen JM, van der Hoek MB, Kong WC, Owens JA, Fischer B, Navarrete Santos A. MicroRNA expression profile during adipogenic differentiation in mouse embryonic stem cells. Physiol Genomics 43: 611– 620, 2011. First published January 18, 2011; doi:10.1152/physiolgenomics.00116.2010.—Pluripotent embryonic stem cells (ESC) have the potential to differentiate into any cell type of the three germ ...

embryonic stem cells (escs) are undifferentiated cells which are typically derived from the inner cell mass of a blastocyst-stage embryo. in culture, these cells have the capacity to self-renew in an undifferentiated state but also may differentiate into cell types representing the three embryonic germ lineages, thus revealing their pluripotent potential. these cells could be of value for creat...

BACKGROUND Pluripotent embryonic stem cells are considered to be an unlimited cell source for tissue regeneration and cell-based therapy. Investigating the molecular mechanism underlying the regulation of embryonic stem cell expansion is thus important. 14-3-3 proteins are implicated in controlling cell division, signaling transduction and survival by interacting with various regulatory protein...

background: recent publications about differentiation of stem cells to germ cells have motivated researchers to make new approaches to infertility. in vitro production of germ cells improves understanding differentiation process of male and female germ cells. due to the problem of using embryonic stem cells (esc), it’s necessary the mentioned cells be replaced with some adult multi-potent stem ...