A culture system for cell suspensions from mouse spleens has been described. The system provides adequate conditions for in vitro immunization on initial exposure to heterologous erythrocytes. The in vitro response closely parallels that observed in vivo with respect to size, early kinetics, antigen dose, and the inhibitory effect of passive antibody. The response of cultured cells differs in t...

Despite its many drawbacks the classic benzidine technique is the method most widely used for determining plasma hemoglobin. An additional disadvantage, reported here, is that the method measures hemoglobin in erythrocytes as well as in plasma. Thus, if plasma specimens are accidentally contaminated with erythrocytes, apparent free hemoglobin in plasma will be artifactually high. Although the p...

Endothelial cell CD36 (glycoprotein IV) has been purified from bovine heart tissue by detergent partitioning and immunoaffinity chromatography. Bovine CD36 differs from human CD36 in its apparent mass (85 versus 88 kDa), primary structure, and immunological cross-reactivity. Of the 18 N-terminal residues identified, 17 conformed to the human CD36 sequence. Mouse monoclonal antibodies E-1 and 8A...

Chronic myelomonocytic leukaemia (CMML) is a disease that borders between myelodyplastic syndrome (MDS) and myeloproliferative neoplasia (MPN). It progressive condition: about 20% of CMML patients progress to secondary acute myeloid (sAML), which has very poor prognosis. In all cases, normal bone marrow function compromised with resulting depletion circulating erythrocytes platelets. We modelle...

Antigenic differences between certain inbred strains of mice which could not be revealed by hemagglutination techniques were readily disclosed by lymphocyte cytotoxicity. With an improved cytotoxicity test lymphotoxic titers were as high as 1:512 with non-hemagglutinating A anti CBA antisera. In other mouse strain combinations, a close parallel of both types of antibody activity was obtained. T...

  Mild anaemia among patients with hyperthyroidism, reduction in intracranial zinc content of erythrocytes and anti- haemolysis of whole blood of patients with Graves, disease under laboratory Condition are the facts which indicate that although erythrocytes are devoid of receptors for thyroid hormones, they are affected by the excess of these hormones. In order to find out the reasons for suc...

TUDIES on the inheritance of nonspecific esterase forms of the house mouse (Mus musculus) have recently been reported from the serum (POPP and POPP 1962; PETRAS 1963), kidney ( RUDDLE and RODERICK 1965), and erythrocytes (PELZER 1965). A brief report (SHAW 1964) indicated inheritable esterase differences in kidney homogenates of the deer mouse, Peromyscus maniculatus, and two rare genetically d...

Src-family kinases play a central role in regulation of hematopoietic cell functions. We found that mouse erythrocytes express the Src-family kinases Fgr and Hck, as well as Lyn. To directly test whether Fgr and Hck play any role in erythrocyte function, we analyzed red cells isolated from fgr-/-, hck-/-, and fgr-/- hck-/- knock-out mice. Mean corpuscular hemoglobin concentration and median den...

Phagocytosis of bacteria stimulates "professional" phagocytes to produce and release endogenous pyrogen (EP), the protein that mediates fever. To determine whether "nonprofessional" phagocytes also have this capacity, mouse and human fibroblasts and HeLa cells were cultured after ingestion of latex or chicken erythrocytes (CE), and EP release into culture supernate measured by mouse assay. No d...

Two mammalian species (porcine and murine) have erythrocytes that are being widely used to study membrane protein synthesis and red cell aging. Erythrocytes of these species however, are significantly smaller than those of the human. Before results obtained from study of these red cells can be applied to human cells, the membrane skeleton of these species must be investigated to determine if th...