objective(s): this study was performed to investigate the effects of mucosal acidification on mrna expression and protein synthesis of cystathionine gamma lyase (cse), cystathionine beta synthase (cbs), and mucosal release of h2s in gastric mucosa in rats. materials and methods:thirty-two rats were randomly assigned into 4 groups (8 in each), including: the control group, hcl (10 mm) treated gr...

AIMS To investigate the role of the matrix metalloproteinases (MMPs) in the connective tissue changes seen in the intestine in Crohn's disease. METHODS Indirect immunofluorescence microscopy using specific antibodies to the MMPs (collagenase, gelatinase A and B, and stromelysin) were used to assess the distribution of these enzymes in normal and diseased intestine. RESULTS In normal intesti...

AIMS To assess changes in indicators of nutrition and iron deficiency as possible non-invasive markers of mucosal recovery in patients with coeliac disease on a gluten free diet. METHODS Concentrations of transthyretin, retinol binding protein, soluble transferrin receptor, IgA anti-gliadin, and IgA anti-transglutaminase, and titres of IgA anti-endomysial antibody were measured in 36 newly di...

BACKGROUND Excessive mucosal generation of cytokines and eicosanoids has been reported in vitro in ulcerative colitis (UC) using traumatising biopsy techniques, and in vivo using time consuming rectal dialysis. AIMS To validate a simple filter paper technique to profile rectal mucosal production of cytokines and eicosanoids in vivo in patients with UC compared with controls. PATIENTS Forty ...

The immunochemical protein content of group II phospholipase A2 (PLA2) and PLA2 enzymatic activity were measured for colonic mucosal biopsy samples obtained from patients with either Crohn's disease of the colon or ulcerative colitis, and control patients without inflammatory bowel disease. Immunoreactive group II PLA2 (IR-PLA2 II) content and PLA2 activity in actively inflamed colonic mucosa o...

Infection with human immunodeficiency virus type 1 (HIV1) has now been reported in virtually every country of the world. Responding to the challenge of this devastating pandemic, basic and clinical investigators in many regions of the world have rapidly advanced our understanding of the biology, immunology, and virology of systemic HIV-1 infection. Although the vast majority of HIV-1 infections...