The LIM domain-binding protein Ldb1 is an essential cofactor of LIM-homeodomain (LIM-HD) and LIM-only (LMO) proteins in development. The stoichiometry of Ldb1, LIM-HD, and LMO proteins is tightly controlled in the cell and is likely a critical determinant of their biological actions. Single-stranded DNA-binding proteins (SSBPs) were recently shown to interact with Ldb1 and are also important in...

Concentrations of 3-chlorophenol and 4-chlorophenol below their minimal inhibitory concentrations were found to increase the permeability of Pseudomonas aeruginosa cells to protons. Levels of such activity were assessed in suspensions of cells prepared from chemostat-grown cultures, limited by either magnesium (Mg-lim) or glucose (G-lim), with the use of five growth rates. Drug concentrations r...

Targeting of proteins to a particular cellular compartment is a critical determinant for proper functioning. LPP (LIM-containing lipoma-preferred partner) is a LIM domain protein that is localized at sites of cell adhesion and transiently in the nucleus. In various benign and malignant tumors, LPP is present in a mutant form, which permanently localizes the LIM domains in the nucleus. Here, we ...

High-speed liquid jets and sprays are complex multiphase flow phenomena with many important industrial applications. Great efforts have been devoted to understand their dynamics since the pioneering work of Rayleigh on low-speed jets. Attempts to use conventional laser optical techniques to provide information about the internal structure of high-speed jets have been unsuccessful owing to the m...

Members of the cysteine- and glycine-rich protein family (CRP1, CRP2, and CRP3) contain two zinc-binding LIM domains, LIM1 (amino-terminal) and LIM2 (carboxyl-terminal), and are implicated in diverse cellular processes linked to differentiation, growth control, and pathogenesis. Here we report the solution structure of full-length recombinant quail CRP2 as determined by multi-dimensional triple...

The possibility to obtain multiphase steels with considerable fractions of retained austenite, which is of key interest for the occurrence of the TRIP effect, is investigated in steels with low amounts of Si and Al. Special emphasis is dedicated to the microstructural observation of this phase, since its volume fraction, distribution and stability, related to the carbon content, will determine ...

Purpose: of this paper is the effect of the soft annealing of initial microstructure of the 6Mn16 steel on the kinetics of the austenite formation during next intercritical annealing. Design/methodology/approach: Analytical TEM point analysis with EDAX system attached to Philips CM20 was used to evaluate the concentration of Mn, Ni and Cr in the microstructure constituents of the multiphase ste...