Transgenic mouse alleles continue to be used heavily in biomedical research (1). Transgenes insert into the genome at random sites, typically in a tandem array of 1 to 20 copies. Both Southern blot analysis and real-time PCR can be used for determining the zygosity of transgenes, but each have practical and technical limitations (2–4). Here we describe a robust, easily implemented method for de...

Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in presence absence ionizing radiation. WTK1 cells contain a p53 mutation, whereas TK6 line has native tumor-suppressor gene. Each isolated pre- post-irradiation (2 3 Gy) analyzed by MLPA. Using probes that target specific regions o...

Abstract Background Molecular genetic testing for the 11p15-associated imprinting disorder Beckwith-Wiedemann syndrome (BWS) is challenging because of molecular heterogeneity and complexity affected imprinted regions. An integrated approach to analyze epigenetic-genetic alterations required accurate diagnosis BWS. Case presentation : We reported a Chinese case with BWS detected by SNP array ana...

Aberrant methylation of promoter CpG islands is causally linked with a number of inherited syndromes and most sporadic cancers, and may provide valuable diagnostic and prognostic biomarkers. In this report, we describe an approach to simultaneous analysis of multiple CpG islands, where methylation-specific oligonucleotide probes are joined by ligation and subsequently amplified by polymerase ch...