PurposeCurrent polymerase chain reaction (PCR) methods for the diagnosis of infections are time consuming and require large sample volume skilled technicians. We developed a novel, easy-to-use, rapid (processing time, 1 minute; total 33 minutes) multiplex real-time PCR test (Direct Strip PCR) that did not DNA extraction to detect 9 pathogens could cause uveitis in 20-μl samples.DesignMulticente...

background and aims: the aim of present study was to investigate the molecular characteristic of staphylococcus aureus to detect mec a gene and to type sccmec in strain isolated from healthy worker at larestan hospital. material and methods: this study was carried out from the 250 nasal swab healthy worker  at larestan hospital. multiplex polymerase chain reaction for meca gene was performed in...

Respiratory viral infections in neonatal intensive care units (NICUs) can cause significant morbidity and mortality. Testing for pathogens is not routinely done many NICUs. Their clinical presentations are unpredictable on infant sepsis evaluation, bacterial cultures frequently sterile. The study aimed to estimate the prevalence of respiratory among neonates clinically diagnosed with using mult...

Cimex lectularius L. (Hemiptera: Cimicidae) is a widespread blood feeding pest of humans around the world, including North America, and has recently undergone a resurgence. A molecular diagnostic technique applying multiplex polymerase chain reaction (PCR) was developed to distinguish bed bug eggs, leg fragments, and degraded samples from other arthropods that frequently occur in human dwelling...

BACKGROUND Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years. OBJECTIVE To assess the value of multiplex ...

The purpose of this study was to develop pneumococcal typing by multiplex PCR and compare it with conventional serotyping by quellung reaction. Pneumococcal strains used in this study included 77 isolates from clinical specimens collected from children at Seoul National University Children's Hospital from 2006 to 2010. These strains were selected as they represented 26 different serotypes previ...

In the laboratory, the department most affected by the introduction of molecular diagnostic tools has been the clinical microbiology laboratory. For organisms such as Clostridium difficile, polymerase chain reaction (PCR) is the gold standard for diagnosis. Incorporation of molecular diagnostic techniques into microbiology laboratories of differing sizes has been hastened by the development of ...

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...