Symptomatic and immunologic responses to allergens from laboratory mice were studied in a research institute. Subjects who had been exposed to mice and 50 unexposed subjects were studied by questionnaire and by prick tests with seven prevalent aeroallergens and allergens from mouse urine and pelts. Of the 121 exposed subjects, 39 (32.2%) had respiratory, ocular, or cutaneous symptoms after expo...

We developed a method for the detection of phosphatase activity using fluorogenic substrates after polyacrylamide gel electrophoresis. When phosphatases such as Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP), protein phosphatase 2C (PP2C), protein phosphatase 5 (PP5), and alkaline phosphatase were resolved by polyacrylamide gel electrophoresis in the absence of SDS and the gel was...

Objectives/Hypothesis The time course of the reinnervation of the paralyzed face after hypoglossal-facial jump nerve suture using electromyography (EMG) was assessed. The relation to the clinical outcome was analyzed. Study Design Retrospective single-center cohort study. Methods Reestablishment of motor units was studied by quantitative EMG and motor unit potential (MUP) analysis in 11 pat...

SUMMARY High–level image analysis is an area which has seen considerable research interest in the past few years. In particular the task of object recognition has been successfully tackled using deformable template models in a Bayesian context. However these models are not being widely used in practise , and one of the reasons for this may be that they do require a number of rather non-intuitiv...

Gary E. Kopec, Member, IEEE, and Mauricio Lomelin, Member, IEEE July 20, 1997 Abstract An approach to supervised training of character templates from page images and unaligned transcriptions is proposed. The template training problem is formulated as one of constrained maximum likelihood parameter estimation within the document image decoding framework. This leads to a three-phase iterative tra...

We present a novel approach to automatic estimation of tempo over time. This method aims at detecting tempo at the tactus level for percussive and nonpercussive audio. The front-end of our system is based on a proposed reassigned spectral energy flux for the detection of musical events. The dominant periodicities of this flux are estimated by a proposed combination of discrete Fourier transform...

Antigens specific to pericentral hepatocytes have been studied in adult mouse liver, during fetal development, and in cultured fetal hepatoblasts. Antibody reactive with glutamine synthetase stained all fetal liver cells but almost all cells lost this antigen after birth; only a single layer of pericentral cells retained it in adulthood. In contrast, monoclonal antibodies to major urinary prote...

BACKGROUND Maximal isometric quadriceps strength deficits have been widely reported in studies of knee osteoarthritis (OA), however little is known about the effect of osteoarthritis knee pain on submaximal quadriceps neuromuscular function. The purpose of this study was to measure vastus medialis motor unit (MU) properties in participants with knee OA, during submaximal isometric contractions....

Odour signals used in competitive and aggressive interactions between males are well studied in the context of sexual selection. By contrast, relatively little is known about comparable signals used by females, despite current interest in the evolution of female ornaments and weaponry. Available evidence suggests that odour signals are important in competitive interactions between female mammal...

Nonradioactive methods for the detection of immobilized protein on Western blots are standard laboratory practice. This is in contrast to nucleic acid blotting, which still commonly involves the use of radioactive probes. Although the nonradioactive detection of antibody (Ab)1 complexes in Western blotting can employ direct detection of Xuorochrome–Ab conjugates, the majority of systems detect ...