In the present work we used a murine mammary cancer model of two related adenocarcinomas with different lung metastasizing abilities, to compare their global gene expression profiles. Clontech Atlas mouse cDNA microarrays of primary cultured tumor cells were employed to identify genes that are modulated in the more metastatic variant MM3 relative to its parental tumor M3. A total of 88 from 1,1...

Mastitis, the inflammation of the mammary gland, is an important disease affecting dairy animals worldwide. The disease is caused by mammary pathogenic bacteria and Escherichia coli are frequently implicated. Virulence factors of mammary pathogenic E. coli are only partially known and intramammary challenge with LPS elicits neutrophil recruitment in experimental bovine and murine mastitis model...

Resistance of hypoxic cells to radiation and chemotherapy remains a major limitation to effective therapy of solid tumors. Misonidazole, a 2nitroimidazole analogue, has been studied extensively as a radiosensitizer of hypoxic cells and has been shown to undergo bioreductive metabolism to exert preferential cytotoxicity against hypoxic cells. We have investi gated the effects of misonidazole on ...

Resistance of hypoxic cells to radiation and chemotherapy remains a major limitation to effective therapy of solid tumors. Misonidazole, a 2-nitroimidazole analogue, has been studied extensively as a radiosensitizer of hypoxic cells and has been shown to undergo bioreductive metabolism to exert preferential cytotoxicity against hypoxic cells. We have investigated the effects of misonidazole on ...

Mitochondria isolated from normal mammary glands taken from lactating and nonlactating C3H mice were compared with those isolated from mammary adenocarcinomas. Functional mitochondria could be obtained from the normal mammary glands only when milk was not present in the tissue prior to homogenization and when bovine serum albumin was added to the isolation medium. Albumin also protected tumor m...

Recombinant interleukin-2 (IL-2)/chemotherapy combinations have recently entered clinical trial. The rationale for sequencing has primarily been empiric or based on in vitro data. To establish in vivo models for chemoimmunotherapy trials, we investigated IL-2 alone and in combination with dacarbazine (DTIC) and adriamycin. IL-2 (as a single agent given i.v. at 1-3 x 10(5) Cetus units once daily...

This case report of multiple mammary tumours in a Spitz bitch revealed the presence of three tumour masses, T-1, T-2 and T-3, of variable size, shape and texture on different mammary glands. Histopathological examination revealed these tumours to be of different histological type (T-1 – fibrosarcoma, T-2 – malignant mixed mammary tumour and T-3 – papillary adenocarcinoma). The mitotic index and...

acterization of the transplantable lactating mammary tumor R3230AB of the Fischer rat was recently reported (11). During that investigation, a slow growing variant neoplastic line occurred in 1 of the animals in which the mammary adenocarcinoma had been implanted for main tenance purposes. Examination of this subline revealed a mammary carcinoma which was comprised primarily of epithelial cells...

Transforming acidic coiled coil 1 (TACC1) is a putative oncogene located within a breast cancer amplicon found on human chromosome 8p11. Although TACC1 has been reported to transform fibroblasts, it is also down-regulated in a subset of mammary tumors treated with anthracyclin. Here, we show that ectopic TACC1 overexpression can cooperate with Ras to induce focus formation in murine fibroblast ...

Mammary carcinoma is one of the most common skin tumors mammary gland area in canine. Fifty cases were reported during period study at Teaching Veterinary Clinical Complex and Department Surgery Radiology. Eighty animals screened for selecting fifty these are subjects present population. The prevalence across age groups highest among 8 to 12 years, followed by years least dogs below years. Bree...