Figure 1: Protein 1CRN, Crambin, is a 46 amino acid protein composed of two alpha helices and a short anti-parallel beta sheet (1(a)). When rigidity analysis is performed on the non-mutated form of Crambin, the majority of the atoms in the proteins core are part of a single large rigid cluster (shown in purple))(1(b)). KINARI-Mutagen was used to generate several in-silico mutants of crambin. Wh...

Mutations arising in vivo in recorder genes of human blood cells provide biomarkers for molecular epidemiology by serving as surrogates for cancer-causing genetic changes. Current markers include mutations of the glycophorin-A (GPA) or hemoglobin (Hb) genes, measured in red blood cells, or mutations of the hypoxanthine-guanine phosphoribosyltransferase (hprt) or HLA genes, measured in T-lymphoc...

In assessing the potential health hazard a given mutagen might present to man, a number of extrapolations must be made from the test systems which are currently available. For the L5178Y mouse lymphoma cell assay system that we use, these extrapolations include: somatic versus germinal cells; single cells versus whole animals; transformed versus "normal" cells; and mouse versus human cells. All...

Mutation breeding is one of the breeding methods used successfully in durum wheat for selecting lines with increased agronomic values. This study was carried out to determine gamma rays effect and interaction between gamma-rays and the genotype of treated variety of winter wheat in mutations induction. We used physical mutagens: gamma-rays in doses 100, 150, 200, 250 Gy. Seeds of eight winter w...

In the aetiology of head and neck squamous cell carcinoma (HNSCC), smoking and heavy alcohol consumption are the main environmental risk factors. The bleomycin (BLM) sensitivity assay is believed to measure environment-related cancer risks, mainly of HNSCC. Previously, we have shown that this method is only moderately sensitive to identify individuals at high risk for developing HNSCC, due to b...