نتایج جستجو برای: mutagenesis analysis
تعداد نتایج: 2845236 فیلتر نتایج به سال:
From population genetics theory, elevating the mutation rate of a large population should progressively reduce average fitness. If the fitness decline is large enough, the population will go extinct in a process known as lethal mutagenesis. Lethal mutagenesis has been endorsed in the virology literature as a promising approach to viral treatment, and several in vitro studies have forced viral e...
macrophomina phaseolina is one of the major yield limiting factors of melons in tropical and subtropical regions. for eco-friendly and effective management of the disease, 24 gamma induced mutants from trichoderma harzianum were evaluated against three isolates of the pathogen representing three geographically different regions viz. khorasan (isolate 1), garmsar (isolate 2) and khuzestan (isola...
A major challenge in the post-genome era of plant biology is to determine the functions of all the genes in the plant genome. A straightforward approach to this problem is to reduce or knock out expression of a gene with the hope of seeing a phenotype that is suggestive of its function. Insertional mutagenesis is a useful tool for this type of study, but it is limited by gene redundancy, lethal...
1Department of Genetics, Cell Biology and Development and Department of Pediatrics, Masonic Cancer Center, University of Minnesota Twin Cities, Minneapolis, MN; 2Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, WI; 3Biostatistics and Bioinformatics, Masonic Cancer Center, University of Minnesota Twin Cities, Minneapolis, MN; 4Department of Hemat...
High-throughput technologies of functional genomics such as T-DNA insertional mutagenesis and microarray expression profiling have been employed to identify genes related to pathogenicity in Magnaporthe oryzae. However, validation of the functions of individual genes identified by these high-throughput approaches is laborious. In this study, we compared two published lists of genes putatively r...
Abstract Teratological spores and pollen are widespread in sediments that record the Permian-Triassic mass extinction. The malformations thought to be result of extreme environmental conditions at time, but mutagenic agents precise timing events remain unclear. We examined abundance teratological sporomorphs metal concentrations a tropical peatland succession southwestern China. find significan...
UNLABELLED Analysis of bacterial gene function commonly relies on gene disruption or replacement followed by phenotypic characterization of the resulting mutant strains. Deletion or replacement of targeted regions is commonly achieved via two homologous recombination (HR) events between the bacterial genome and a nonreplicating plasmid carrying DNA fragments flanking the region to be deleted. T...
The effects of gamma radiation are investigated by studying seed germination, survival rate and morphological characteristics Stevia rebaudiana. Improvement stevia plant through mutagenesis could be one the ways to produce with delay flowering.
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