A mitoxantrone-resistant human MCF-7 breast cancer subline (MCF/MX) which is approximately 4000-fold resistant to mitoxantrone was isolated by serial passage of the parental wild-type MCF-7 cells (MCF/WT) in stepwise increasing concentrations of drug. MCF/MX cells were also approximately 10-fold cross-resistant to doxorubicin and etoposide but were not cross-resistant to vinblastine. Intracellu...

but only rarely is a new type of meteorite discovered. Last week in Science, Brown et al. described what might be such an event with the orbit, fall and recovery of numerous fragments of a meteorite in the Canadian Yukon. Named after the frozen lake on which it fell, the Tagish Lake meteorite is a chondrite, which means it is a stony aggregate of matter from the early Solar System. It is descri...

hydrotactic. Alternatively, cells may rely on sensing parameters that vary with desiccation, such as salinity, temperature or the presence of other chemical species. We have also seen this behaviour in other desert-soil cyanobacteria — for example, the common Microcoleus vaginatus . If it is widespread among prokaryotes, this response may be crucial for survival and dispersal in terrestrial env...

• State-F: Forward Outliers p(dx|Zx,mx = 1) = Uf (dx|Zx) = Uf · 1[dtxZx]. For the purpose to combine all the three states into a united model and describe the overall likelihood that the input depth samples fit the current static structure, we use a mixture model similar to the Gaussian Mixture Model [1]. Together with ...

Apoptosis is implicated in the life cycle of the malaria parasite in mosquitoes. The genome project for the primary malaria vector Anopheles gambiae showed a significant expansion of the inhibitor of apoptosis protein (IAP) and caspase gene families in comparison with Drosophila. However, because of extensive sequence divergence, no orthologue was identified for the reaper/grim-like IAP antagon...

The oxidative degradation of Procion Red MX-5B by solar Fenton’s has been investigated using sunlight as irradiation source in presence of hydrogen peroxide and Fe2+. Maximum decolorization (99%) and COD removal (97%) for MX-5B was obtained at H2O2 dose of 16.6 mM and at 30 min irradiation time. Fe2+ dose of 0.36 mM was optimum for Procion Red MX-5B degradation. The solar degradation mechanism ...

OBJECTIVE This study intended to observe the effects of methoxyamine (Mx) on cytotoxic effects and DNA damage caused by 5-Fluorouracil (5-FU) in combination with gamma radiation in a human colon cancer cell line, HT29. MATERIALS AND METHODS In this experimental study, HT29 cells were cultured as a monolayer and treated with different concentrations of 5-FU along with 1 mM Mx for 24 hours. Nex...

Let (X , x0) be any one–pointed compact connected Riemann surface of genus g, with g ≥ 3. Fix two mutually coprime integers r > 1 and d. Let MX denote the moduli space parametrizing all logarithmic SL(r, C)–connections, singular over x0, on vector bundles over X of degree d. We prove that the isomorphism class of the variety MX determines the Riemann surface X uniquely up to an isomorphism, alt...

We cloned and sequenced a 2.35-kilobase EcoRI fragment of genomic DNA from a local freshwater fish (Perca fluviatilis) that strongly hybridized to probes derived from the murine influenza virus resistance gene Mx. The cloned fish DNA contained blocks of sequences related to Mx gene exons 3 to 8, which appeared to represent exons of a bona fide fish gene because they were separated by intron seq...

In this study we demonstrate that the different substrate recognition properties of bacterial and human AP endonucleases might be used to quantify and localize apurinic (AP) sites formed in DNA in vivo. By using a model oligonucleotide containing a single AP site modified with methoxyamine (MX), we show that endonuclease III and IV of E. coli are able to cleave the alkoxyamine-adducted site whe...